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This study exposed murine long bone osteocyte\u2010Y4 (MLO\u2010Y4) cells to DEX below normoxic or hypoxic circumstances and found that cell autophagy have been reduced. At the same time, flow cytometry analysis showed increased apoptosis, which was more pronounced in hypoxic environments. Recent research also claimed that GC induces osteoporosis after osteocyte apoptosis, and subsequent microfractures lead to ischemia and hypoxia of the femoral head, resulted in GIOFH. Presently, we found that both mitophagy\u2010related protein hypoxia\u2010inducible factor\u20101\u03b1 (HIF\u20101\u03b1) and BNIP3 were up\u2010regulated in the hypoxic environment, and their expression was down\u2010regulated when exposed to DEX. Besides, we demonstrated that overexpressing HIF\u20101\u03b1 resisted DEX\u2010induced apoptosis in a hypoxic environment. Here, we demonstrated that overexpression of HIF\u20101\u03b1, through its downstream marker BNIP3, reduced the suppression of DEX on mitophagy induced by hypoxia and protected bone cells from apoptosis. Also, these findings may provide a direction of the promising application for better GIOFH treatment shortly.<\/jats:p>","DOI":"10.1002\/tox.23327","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T14:09:38Z","timestamp":1627308578000},"page":"2123-2137","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Overexpression of <scp>HIF<\/scp>\u20101\u03b1 enhances the protective effect of mitophagy on steroid\u2010induced osteocytes apoptosis"],"prefix":"10.1002","volume":"36","author":[{"given":"Ke","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Orthopaedics of the First Affiliated Hospital Medical School, Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery Hong Hui Hospital, Xi'an Jiaotong University  Xi'an 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