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In this study, zebrafish were exposed to chlorobromoisocyanuric acid (0, 30, 40, and 50\u2009mg\/L) from 10 to 96\u2009h post\u2010fertilization (hpf). We found a significant reduction in immune cell numbers (neutrophils and macrophages) and the area of thymus at 96\u2009hpf. The expression of immune\u2010related genes and pro\u2010inflammatory cytokines genes were upregulated. Besides, chlorobromoisocyanuric acid triggered neutrophils cell apoptosis. The mRNA and protein levels of pro\u2010apoptotic p53 pathway and the Bax\/Bcl\u20102 ratio further indicated the underlying mechanism. Furthermore, the oxidative stress was observed that the accumulation of reactive oxygen species and malondialdehyde significantly increased. Subsequently, the antioxidant agent astaxanthin significantly attenuated the level of oxidative stress and the dysregulation of inflammatory response. In summary, our results showed that chlorobromoisocyanuric acid induced developmental defects and immunotoxicity of zebrafish, partly owing to oxidative stress and cell apoptosis.<\/jats:p>","DOI":"10.1002\/tox.23413","type":"journal-article","created":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T11:45:58Z","timestamp":1638186358000},"page":"468-477","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of chlorobromoisocyanuric acid on embryonic development and immunotoxicity of zebrafish"],"prefix":"10.1002","volume":"37","author":[{"given":"Yi","family":"Liu","sequence":"first","affiliation":[{"name":"College of life sciences Jiangxi Normal university  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7961-8131","authenticated-orcid":false,"given":"Jing","family":"Guo","sequence":"additional","affiliation":[{"name":"College of life sciences 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