{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T04:16:09Z","timestamp":1780805769300,"version":"3.54.1"},"reference-count":47,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T00:00:00Z","timestamp":1655510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013061","name":"Jilin Scientific and Technological Development Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013061","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dibutyl phthalate (DBP) is a plasticizer widely used in daily production, which causes serious environmental pollution, and damage to brain, liver, kidney, and lung by producing excessive reactive oxygen species (ROS) after entering the body. DBP can also cause skeletal dysplasia, but it is unclear whether ROS is involved. In addition, overproduction of ROS can activate mitophagy, which is an important mechanism for regulating mitochondrial quality and cell homeostasis. In order to investigate whether DBP can damage MC3T3\u2010E1 cells (osteoblast cell line) and whether ROS and mitophagy are involved, DBP toxicity experiment, Parkin gene silencing experiment, and N\u2010acetylcysteine (NAC) intervention experiment were performed on MC3T3\u2010E1 cells in turn. First, we found that DBP caused MC3T3\u2010E1 cell viability decline and osteogenic dysfunction, accompanied by the overproduction of ROS and the activation of mitophagy. Then, we found that silencing Parkin expression alleviated DBP\u2010induced apoptosis and osteogenic dysfunction of MC3T3\u2010E1 cells. In addition, NAC treatment inhibited the PINK1\/Parkin\u2010mediated mitophagy and alleviated the apoptosis and osteogenic dysfunction of MC3T3\u2010E1 cells caused by DBP. Our research results showed that DBP could cause MC3T3\u2010E1 cell damage by increasing ROS to promote the PINK1\/Parkin\u2010mediated mitophagy.<\/jats:p>","DOI":"10.1002\/tox.23600","type":"journal-article","created":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T08:54:42Z","timestamp":1655542482000},"page":"2341-2353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Dibutyl phthalate causes<scp>MC3T3\u2010E1<\/scp>cell damage by increasing<scp>ROS<\/scp>to promote the<scp>PINK1<\/scp>\/Parkin\u2010mediated mitophagy"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8852-7461","authenticated-orcid":false,"given":"Yilong","family":"Cui","sequence":"first","affiliation":[{"name":"Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine Northeast Agricultural University Harbin 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