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Liver MC accumulation and structural change are intensely studied, but the functional hepatic enzymes and energy metabolism have received little attention. This study investigated the liver and hepatocyte structures and the activity of key hepatic functional enzymes with emphasis on energetic metabolism changes after subchronic fish exposure to cyanobacterial crude extract (CE) containing MC. The Neotropical erythrinid fish, <jats:italic>Hoplias malabaricus<\/jats:italic>, were exposed intraperitoneally to CE containing 100\u2009\u03bcg\u2009MC\u2010LR\u2009eq\u2009kg<jats:sup>\u22121<\/jats:sup> for 30\u2009days and, thereafter, the plasma, liver, and white muscle was sampled for analyses. Liver tissue lost cellular structure organization showing round hepatocytes, hyperemia, and biliary duct obstruction. At the ultrastructural level, the mitochondria and the endoplasmic reticulum exhibited disorganization. Direct and total bilirubin increased in plasma. In the liver, the activity of acid phosphatase (ACP) increased, and the aspartate aminotransferase (AST) decreased; AST increased in plasma. Alkaline phosphatase (ALP) and alanine aminotransferase (ALT) were unchanged in the liver, muscle, and plasma. Glycogen stores and the energetic metabolites as glucose, lactate, and pyruvate decrease in the liver; pyruvate decreased in plasma and lactate decreased in muscle. Ammonia levels increased and protein concentration decreased in plasma. CE alters liver morphology by causing hepatocyte intracellular disorder, obstructive cholestasis, and dysfunction in the activity of key liver enzymes. The increasing energy demand implies glucose mobilization and metabolic adjustments maintaining protein preservation and lipid recruitment to supply the needs for detoxification allowing fish survival.<\/jats:p>","DOI":"10.1002\/tox.23628","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T07:39:20Z","timestamp":1659512360000},"page":"2683-2691","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Liver dysfunction and energy storage mobilization in tra\u00edra, <i>Hoplias malabaricus<\/i> (Teleostei, Erythrinidae) induced by subchronic exposure to toxic cyanobacterial crude extract"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0464-6848","authenticated-orcid":false,"given":"Marcelo Gustavo","family":"Paulino","sequence":"first","affiliation":[{"name":"Department of Physiological Sciences Federal University of S\u00e3o Carlos  S\u00e3o Carlos SP 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