{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T23:28:29Z","timestamp":1792106909624,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T00:00:00Z","timestamp":1659398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004606","name":"Chang Gung Medical Foundation","doi-asserted-by":"publisher","award":["CMRPG6F0141"],"award-info":[{"award-number":["CMRPG6F0141"]}],"id":[{"id":"10.13039\/501100004606","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Colorectal cancer is the third leading cause of cancer death in Taiwan. Current treatments involve combination of surgical resection, radiation, and chemotherapy. These treatments have demonstrated to increased five\u2010year survival of a patient with colorectal cancer. However, metastasis is a major capability of cancer cells that causes poor prognosis, recurrence, and even death. Epidemiological and clinical studies have suggested the use of non\u2010steroidal anti\u2010inflammatory drugs (NSAIDs) as an effective class of compounds to prevent colon cancer. Parecoxib is an NSAID and the only parenterally administered selective cyclooxygenase (COX)\u20102 inhibitor. In this study, we evaluated whether parecoxib inhibits the metastasis of DLD\u20101 human colon cancer cells, a COX\u20102 null cell line, and the underlying mechanism. Cell migration of the DLD\u20101 cells was significantly inhibited by parecoxib treatment as shown by the Transwell migration assay. This enhanced anti\u2010migration effect was correlated with the attenuated phosphorylation of Akt, expression of vimentin (a mesenchymal marker), and \u03b2\u2010catenin, and corresponded with the upregulated GSK3\u03b2 and E\u2010cadherin (an epithelial marker). These findings suggested that parecoxib could inhibit the epithelial\u2010mesenchymal transition (EMT) and metastasis in human colon cancer cells by downregulating \u03b2\u2010catenin. Thus, parecoxib could provide a novel prospective strategy for a combination treatment with chemotherapeutic drugs against metastasis of human colon cancer.<\/jats:p>","DOI":"10.1002\/tox.23631","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T19:54:59Z","timestamp":1659470099000},"page":"2718-2727","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Parecoxib expresses anti\u2010metastasis effect through inhibition of epithelial\u2010mesenchymal transition and the Wnt\/\u03b2\u2010catenin signaling pathway in human colon cancer <scp>DLD<\/scp>\u20101 cell line"],"prefix":"10.1002","volume":"37","author":[{"given":"Chung Hang","family":"Wong","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Chang Gung Memorial Hospital at ChiaYi  Chia\u2010Yi Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan\u2010Ling","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Chang Gung Memorial Hospital at ChiaYi  Chia\u2010Yi Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fung\u2010Jou","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Medicine Chung Shan Medical University  Taichung Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Wen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences National Chiayi University  Chiayi City Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyun\u2010Yu","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Physical Medicine and Rehabilitation Chang Gung Memorial Hospital  Chia\u2010Yi Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0578-7116","authenticated-orcid":false,"given":"Ching\u2010Hsein","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences National Chiayi University  Chiayi City Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.06.178"},{"key":"e_1_2_7_3_1","first-page":"6130","article-title":"Critcal factors in the biology of human cancer metastasis: twenty\u2010eighth G.H.A. 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