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Hence, the effects of the traditional Chinese medicine Qiangjing tablet (QJP) on sperm quality and BTB alterations induced by Cd in mouse testes were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adult KM mice challenged with Cd chloride were examined, QJP was administered to mice as an oral drug by gavage, and the experiments lasted 2\u2009weeks. Testicular and epididymal weights, sperm quality, anti\u2010sperm antibodies (AsAb), hormone levels, and histology were evaluated. Changes in the levels of N\u2010cadherin, occludin, ZO\u20101, claudin\u201011, F\u2010actin, and \u03b2\u2010tubulin and their mRNAs were evaluated. The effects of QJP on the PI3K\/Akt\/Rictor pathway were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CdCl<jats:sub>2<\/jats:sub> decreased reproductive organ weight, sperm quality, and testosterone (T) levels; increased AsAb, follicle\u2010stimulating hormone (FSH), and luteinizing hormone (LH) levels; induced structural damage in testicles with BTB disruption; increased BTB permeability; and decreased N\u2010cadherin, occludin, ZO\u20101, claudin\u201011, F\u2010actin, and \u03b2\u2010tubulin expression. After treatment, QJP blocked the effects of Cd on reproductive organ weight, sperm quality, and T; mitigated germinal epithelium compartment alterations; decreased AsAb, FSH, and LH levels; and preserved BTB ultrastructure and function. In addition, QJP induced increases in N\u2010cadherin, occludin, ZO\u20101, claudin\u201011, F\u2010actin, and \u03b2\u2010tubulin levels and the expression of their mRNAs through the PI3K\/Akt\/Rictor pathway. After the application of JRAB2011, the levels of a specific mTORC2 suppressor, Rictor, and the BTB\u2010protective effect of QJP were greatly reduced.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We demonstrated the effect of QJP against Cd\u2010induced damage to the BTB, and the results indicate that QJP may play a significant role in opposing the effects of Cd through the PI3K\/Akt\/Rictor pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/tox.23706","type":"journal-article","created":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T15:56:31Z","timestamp":1668268591000},"page":"591-603","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The traditional Chinese medicine Qiangjing tablet prevents <scp>blood\u2010testis<\/scp> barrier injury induced by <scp>CdCl<sub>2<\/sub><\/scp> through the <scp>PI3K<\/scp>\/Akt\/Rictor signaling 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