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Previously, we have determined that ferulic acid (FA) protected hair cells against serial concentrations of neomycin\u2010induced ototoxic damage. The aim of the present study is to assess the mechanism and effects of FA on neomycin\u2010induced hair cells loss and impact on mechanosensory\u2010mediated behaviors alteration using transgenic zebrafish (<jats:italic>pvalb3b<\/jats:italic>: <jats:italic>TagGFP<\/jats:italic>). We first identified the optimal protective condition as pre\/co\u2010treatment method in early fish development. Pretreatment of the larvae with FA significantly protected against neomycin\u2010induced hair cells loss through preventing neomycin passed through the cytoplasm of hair cells, and subsequently decreased reactive oxygen species production and TUNEL signals in 4\u00a0day post\u2010fertilization (dpf) transgenic zebrafish larvae. Moreover, preservation of functional hair cells correlated directly with rescue of the altered swimming behavior, indicates FA pretreatment protects against neomycin ototoxic damage in 7\u2010dpf transgenic zebrafish larvae. Together, our findings unravel the otoprotective role of FA as an effective agent against neomycin\u2010induced ototoxic effects and offering the theoretical foundation for discovering novel candidates for hearing protection.<\/jats:p>","DOI":"10.1002\/tox.23707","type":"journal-article","created":{"date-parts":[[2022,11,11]],"date-time":"2022-11-11T13:06:55Z","timestamp":1668172015000},"page":"604-614","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Protective mechanism of ferulic acid against neomycin\u2010induced ototoxicity in zebrafish"],"prefix":"10.1002","volume":"38","author":[{"given":"Hsin\u2010Lin","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences Chung Shan Medical University  Taichung Taiwan"},{"name":"Department of Medical Research Chung Shan Medical University Hospital  Taichung 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