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A bioinformatics analysis was performed to analyze the expression and prognostic significance of DLGAP5 in EC tissues using TCGA, CPTAC, Human Protein Atlas, and GSE63678 databases, UALCAN web tool, and the Kaplan\u2013Meier plotter. Effects of DLGAP on EC cell malignant properties were evaluated by CCK\u20108, flow cytometry analysis, TUNEL assay, caspase\u20103 activity assay, and Transwell invasion assay. The expression of DLGAP5, Wnt3, c\u2010Myc, Ki67, and cleaved caspase\u20103 was detected by western blot analysis. DLGAP5 was highly expressed and correlated with poor prognosis in EC patients. DLGAP5 knockdown inhibited proliferation and invasion, triggered apoptosis, and increased caspase\u20103 activity in EC cells. Additionally, DLGAP5 knockdown inactivated the Wnt\/\u03b2\u2010catenin signaling pathway in EC cells. Moreover, \u03b2\u2010catenin overexpression abolished the effects of DLGAP5 knockdown on the malignant phenotypes of EC cells. DLGAP5 silencing suppressed the malignant properties in EC cells by inactivating the Wnt\/\u03b2\u2010catenin pathway.<\/jats:p>","DOI":"10.1002\/tox.23720","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T19:03:06Z","timestamp":1669921386000},"page":"685-693","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["<scp>DLGAP5<\/scp> knockdown inactivates the Wnt\/\u03b2\u2010catenin signal to repress endometrial cancer cell malignant activities"],"prefix":"10.1002","volume":"38","author":[{"given":"Ruipu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Obstetrics Fokind Hospital Affiliated to Tibet University  Lhasa Tibet China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Fokind Hospital Affiliated to Tibet University  Lhasa Tibet 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