{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:24:22Z","timestamp":1791289462394,"version":"4.3.0"},"reference-count":62,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T00:00:00Z","timestamp":1678147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Methylmercury (MeHg) is a highly poisonous form of mercury and a risk factor for kidney impairment in humans that currently has no effective means of therapy. Ferroptosis is a non\u2010apoptotic metabolic cell death linked to numerous diseases. It is currently unknown whether ferroptosis takes part in MeHg\u2010induced kidney damage. Here, we established a model of acute kidney injury (AKI) in mice by gavage with different doses of MeHg (0, 40, 80, 160\u2009\u03bcmol\/kg). Serological analysis revealed elevated levels of UA, UREA, and CREA; H&amp;E staining showed variable degrees of renal tubule injury; qRT\u2010PCR detection displayed increased expression of KIM\u20101 and NGAL in the groups with MeHg treatment, indicated that MeHg successfully induced AKI. Furthermore, MDA levels enhanced in renal tissues of mice with MeHg exposure whereas GSH levels decreased; ACSL4 and PTGS2 nucleic acid levels elevated while SLC7A11 levels reduced; transmission electron microscopy illustrated that the density of the mitochondrial membrane thickened and the ridge reduced considerably; protein levels for 4HNE and TfR1 improved since GPX4 levels declined, all these results implying the involvement of ferroptosis as a result of MeHg exposure. Additionally, the observed elevation in the protein levels of NLRP3, p\u2010p65, p\u2010p38, p\u2010ERK1\/2, and KEAP1 in tandem with downregulated Nrf2 expression levels indicate the involvement of the NF\u2010\u03baB\/NLRP3\/MAPK\/Nrf2 pathways. All the above findings suggested that ferroptosis and the NF\u2010\u03baB\/NLRP3\/MAPK\/Nrf2 pathways are implicated in MeHg\u2010induced AKI, thereby providing a theoretical foundation and reference for future investigations into the prevention and treatment of MeHg\u2010induced kidney injury.<\/jats:p>","DOI":"10.1002\/tox.23770","type":"journal-article","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T05:44:25Z","timestamp":1678167865000},"page":"1372-1383","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Investigations into ferroptosis in methylmercury\u2010induced acute kidney injury in mice"],"prefix":"10.1002","volume":"38","author":[{"given":"Yingrong","family":"Ye","sequence":"first","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichun","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanpeng","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwu","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youpeng","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peixuan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhikai","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengtao","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5918-3644","authenticated-orcid":false,"given":"Ershun","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Engineering Foshan University  Foshan Guangdong Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,7]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","article-title":"Influence of dietary protein levels on the acute toxicity of methylmercury in mice","volume":"42","author":"Adachi T","year":"1996","journal-title":"Jpn J Tox Env Health"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2020.111686"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0197220"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2013.01.007"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.02110s111"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2019.125586"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1289\/EHP3904"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3961\/jpmph.2012.45.6.353"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-03777-6_1"},{"issue":"2","key":"e_1_2_8_11_1","first-page":"266","article-title":"Renal PORPHYRINURIA during chronic methyl mercury exposure","volume":"90","author":"Woods JS","year":"1977","journal-title":"J Lab Clin Med"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-017-0905-2"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2011.01.002"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-36.3.423"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfp219"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1382-6689(96)00070-1"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.09.017"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.05.005"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1590\/1806-9282.66.s1.68"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)32563-2"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23031"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23467"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23376"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23126"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.590226"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23057"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2019.151058"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23388"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23509"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23615"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-020-01932-z"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2239"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2238"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23662"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2020.12.020"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15519"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.54113"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.45050"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.62903"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-019-02335-7"},{"key":"e_1_2_8_42_1","article-title":"The cross\u2010link between Ferroptosis and kidney diseases","volume":"2021","author":"Wang J","year":"2021","journal-title":"Oxid Med Cell Longev"},{"issue":"6","key":"e_1_2_8_43_1","first-page":"552","article-title":"Research on acupuncture in regulating the inflammatory reaction in anti\u2010ischemia\u2010reperfusion injury","volume":"42","author":"Feng J\u2010J","year":"2017","journal-title":"Zhen Ci Yan Jiu = Acupunct Res"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1515\/reveh-2020-0026"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2019.04.008"},{"issue":"7","key":"e_1_2_8_46_1","first-page":"980","article-title":"Inhibition of mitochondrial reactive oxygen species reduces high glucose\u2010induced pyroptosis and ferroptosis in H9C2 cardiac myocytes. Nan fang yi ke da xue xue bao =","volume":"41","author":"Wang J","year":"2021","journal-title":"J Southern Med Univ"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/oncsis.2017.65"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.07.078"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23586"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002040050104"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1991.312"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28251"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2010.04.007"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.09.008"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2019.121390"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23713"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.782557"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2019.01.006"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.121110"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1FO01036K"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101107"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20010039"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2019.06.010"}],"container-title":["Environmental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.23770","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/tox.23770","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.23770","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T10:16:38Z","timestamp":1764238598000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tox.23770"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,7]]},"references-count":62,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1002\/tox.23770"],"URL":"https:\/\/doi.org\/10.1002\/tox.23770","archive":["Portico"],"relation":{},"ISSN":["1520-4081","1522-7278"],"issn-type":[{"value":"1520-4081","type":"print"},{"value":"1522-7278","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,7]]},"assertion":[{"value":"2022-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}