{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T10:20:21Z","timestamp":1764238821791,"version":"3.46.0"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T00:00:00Z","timestamp":1680048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073582","82103876"],"award-info":[{"award-number":["82073582","82103876"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hydroquinone (HQ), one of the metabolites of benzene in humans, has significant hepatotoxic properties. Chronic exposure to HQ can lead to leukemia. In a previous study by this group, we constructed a model of malignant transformation of human lymphoblastoid cells (TK6) induced by chronic exposure to HQ with significant subcutaneous tumorigenic capacity in nude mice. miR\u201092a\u20103p is a tumor factor whose role in HQ\u2010induced malignant transformation is not yet clear. In the present study, raw signal analysis and dual\u2010luciferase reporter gene results suggested that miR\u201092a\u20103p could target and regulate TOB1, and the expression level of miR\u201092a\u20103p was significantly upregulated in the long\u2010term HQ\u2010induced TK6 malignant transformation model, while the anti\u2010proliferative factor TOB1 was significantly downregulated. To investigate the mechanism behind this, we inhibited miR\u201092a\u20103p in a malignant transformation model and found a decrease in cell viability, a decrease in MMP\u20109 protein levels, a G2\/M phase block in the cell cycle, and an upregulation of the expression of G2\/M phase\u2010related proteins cyclinB1 and CDK1. Inhibition of miR\u201092a\u20103p in combination with si\u2010TOB1 restored cell viability, inhibited cyclin B1 and CDK1 protein levels, and attenuated the G2\/M phase block. Taken together, miR\u201092a\u20103p reduced the cell proliferation rate of HQ19 and caused cell cycle arrest by targeting TOB1, which in turn contributed to the altered malignant phenotype of the cells. This study suggests that miR\u201092a\u20103p is likely to be a biomarker for long\u2010term HQ\u2010induced malignant transformation of TK6 and could be a potential therapeutic target for leukemia caused by long\u2010term exposure to HQ.<\/jats:p>","DOI":"10.1002\/tox.23775","type":"journal-article","created":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T09:49:41Z","timestamp":1680083381000},"page":"1420-1430","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of mir\u201092a\u20103p on hydroquinone induced changes in human lymphoblastoid cell cycle and apoptosis"],"prefix":"10.1002","volume":"38","author":[{"given":"Mei","family":"Xuan","sequence":"first","affiliation":[{"name":"The First Dongguan Affiliated Hospital Guangdong Medical University  Dongguan 523808 China"},{"name":"Dongguan Key Laboratory of Environmental Medicine, School of Public Health Guangdong Medical University  Dongguan 523808 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