{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T14:47:42Z","timestamp":1784299662021,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Exosomes are applied as biomarkers in several diseases according to their disease\u2010specific profiles. However, the exosomes effects in functional dyspepsia (FD) are still fragmentary. Here we examined the role of Eosinophil and mast cell derived\u2010exosomes in FD progression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fifty FD subjects and age\u2010 and sex\u2010matched healthy controls were included in this retrospective cohort study. Duodenal mucosa and gastric juice were collected to analyze molecular difference. Eosinophil and mast cell were evaluated by immunofluorescence and microarray was subjected to examine the expression levels of NEAT1, miR\u2010211\u20105p, and glial cell line\u2010derived neurotrophic factor (GDNF), which were subsequently were tested by quantitative reverse transcription PCR (RT\u2010qPCR) validation cohorts. CCK\u20108 assays, and wound healing assays were used to evaluate integrity of intestinal mucosal barrier in vitro. Rats' weights and gastric emptying rates were used as evaluation of FD severity in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Eosinophil and mast cell were enriched and secreted more exosomes in duodenal mucosa of FD patients. We identified differential lncRNAs that were consistently and significantly up regulated in FD cases. Of these, NEAT1 was further validated by RT\u2010qPCR and had closely relationship with GDNF. MiR\u2010211\u20105p level was found to be reduced in FD and negatively related with NEAT1 and GDNF. Furthermore, NEAT1and GDNF relived FD while miR\u2010211\u20105p made symptoms worse. The NEAT1\/miR\u2010211\u20105p\/GDNF axis had a good predictive ability for FD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The NEAT1\/miR\u2010211\u20105p\/GDNF could be a potential FD biomarker.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/tox.23895","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T07:49:32Z","timestamp":1689752972000},"page":"2595-2607","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Eosinophil and mast cell\u2010derived exosomes promote integrity of intestinal mucosa via the\n                    <scp>NEAT1<\/scp>\n                    \/\n                    <scp>miR<\/scp>\n                    \u2010211\u20105p\/\n                    <scp>glial cell line\u2010derived neurotrophic factor<\/scp>\n                    axis in duodenum"],"prefix":"10.1002","volume":"38","author":[{"given":"Jue","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6874-3874","authenticated-orcid":false,"given":"Bo","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Chongqing Medical University  Chongqing 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