{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T16:10:19Z","timestamp":1747152619030,"version":"3.40.5"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T00:00:00Z","timestamp":1692144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["20210101451JC"],"award-info":[{"award-number":["20210101451JC"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Formaldehyde (FA) is associated with the occurrence of leukemia, and oxidative stress is considered to be a major reason. As an endogenous biomarker of oxidative stress, few studies focus on the relationship between peroxiredoxin III (PrxIII) and FA toxicity. Our previous research observed high expression of PrxIII occurred in the process of apoptosis of bone marrow cells (BMCs) induced by FA, however the exact mechanism is unclear. Therefore, this paper aimed to explore the possible association between FA toxicity and PrxIII gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We first, used a Cell Counting Kit\u20108 (CCK\u20108) to detect the viability of BMCs after they were exposed to different doses of FA (50, 100, 200\u2009\u03bcmol\/L) for different exposure time (12, 24, 48\u2009h), then chose 24\u2009h as an exposure time to detect the expression of PrxIII for exposing different doses of FA by Quantitative reverse transcription\u2010PCR (qRT\u2010PCR) and Western blot analysis. Based on our preliminary experimental results, we chose 100\u2009\u03bcmol\/L FA as an exposure dose to expose for 24\u2009h, and used a small interfering RNA (siRNA) to silenced PrxIII to examine the cell viability by CCK\u20108, reactive oxygen species (ROS) level by DCFH\u2010DA, apoptosis by Annexin V\/PI double staining and cell cycle by flow cytometry (FCM) so as to explore the possible regulatory effect of PrxIII silencing on FA\u2010induced bone marrow toxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High expression of PrxIII occurred in the process of FA\u2010induced oxidative stress. Silencing of PrxIII prevented FA from inducing oxidative stress, thus increasing cell viability, decreasing ROS level, rescuing G<jats:sub>0<\/jats:sub>\u2013G<jats:sub>1<\/jats:sub> and G<jats:sub>2<\/jats:sub>\u2013M arrest, and reducing cell apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PrxIII silencing might be a potential target for alleviating FA\u2010induced oxidative damage.<\/jats:p><\/jats:sec>","DOI":"10.1002\/tox.23915","type":"journal-article","created":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T05:50:34Z","timestamp":1692424234000},"page":"2836-2844","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Silencing of peroxiredoxin <scp>III<\/scp> inhibits formaldehyde\u2010induced oxidative damage of bone marrow cells in <scp>BALB\/c<\/scp> mice"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8731-4170","authenticated-orcid":false,"given":"Guangyan","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Preventive Medicine, School of Public Health Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangfu","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine, School of Public Health Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine, School of Public Health Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine, School of Public Health Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.21910"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.20547"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/19338244.2020.1816883"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22982"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22703"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22010"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408444.2017.1301878"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40557-018-0218-z"},{"issue":"1","key":"e_1_2_11_10_1","first-page":"78","article-title":"Formaldehyde induces DNA strand breaks on spermatozoa and lymphocytes of Wistar rats","volume":"51","author":"Jimenez\u2010Villarreal J","year":"2017","journal-title":"Tsitol Genet"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-019-06986-y"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2014.15.13.5253"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-015-9309-6"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2021.103134"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327117728385"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7167"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.15430\/JCP.2019.24.2.65"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4334"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370216669834"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.396"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.21580"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7422"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184407"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1248\/jhs.55.739"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2013.1370"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-015-1393-6"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2008.08.021"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-015-1916-3"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2019.05.086"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-016-1760-5"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2020.106050"},{"key":"e_1_2_11_32_1","unstructured":"IARC.Monographs on the evaluation of carcinogenic risks to humans \u2013formaldehyde. 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