{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:53:46Z","timestamp":1790916826898,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30972510"],"award-info":[{"award-number":["30972510"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Benzene exposure inhibits the hematopoietic system and leads to the occurrence of various types of leukemia. However, the mechanism underlying the hematotoxicity of benzene is still largely unclear. Emerging evidence has shown that exosomes are involved in toxic mechanisms of benzene. To understand the effect of 1,4\u2010benzoquinone (PBQ; an active metabolite of benzene in bone marrow) on the exosomal release characteristics and role of exosomal secretion in PBQ\u2010induced cytotoxicity. Exosomes were isolated from PBQ\u2010treated HL\u201060 cells, purified by ultracentrifugation, and verified by transmission electron microscopy, nanoparticle tracking analysis and the presence of specific biomarkers. Our results showed that PBQ increased exosomal secretion in a dose\u2010dependent manner, reaching a peak in 3\u2009h at 10\u2009\u03bcM PBQ treatment and then slowly decreasing in HL\u201060 cells. The exosomes contained miRNAs, which have been reported to be associated with benzene exposure or benzene poisoning. In particular, mir\u201034a\u20103p and mir\u201034A\u20105p were enriched in exosomes derived from PBQ\u2010treated cells. In addition, the inhibition of exosomal release by GW4869 (an inhibitor of exosomal release) exacerbated PBQ\u2010induced cytotoxicity, including increased intracellular reactive oxygen species levels, decreased mitochondrial membrane potential, and increased the apoptosis rate. Our findings illustrated that exosomes secretion plays an important role in antagonizing PBQ\u2010induced cytotoxicity and maintaining cell homeostasis.<\/jats:p>","DOI":"10.1002\/tox.23944","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T01:25:52Z","timestamp":1697073952000},"page":"1099-1106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Blocking exosomal secretion aggravated 1,4\u2010benzoquinone\u2010induced cytotoxicity"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1597-5516","authenticated-orcid":false,"given":"Qianqian","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Preventive Medicine, School of Public Health and Management Wenzhou Medical University  Wenzhou People's Republic of China"},{"name":"Department of Medicine Shandong Xiandai University  Jinan Shandong People's Republic of 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