{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T10:44:43Z","timestamp":1785840283454,"version":"3.56.0"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T00:00:00Z","timestamp":1703116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Postmenopausal osteoporosis (PMOP) poses a significant threat to women's health worldwide. Eupatilin is a key bioactive component of the Chinese herbal medicine <jats:italic>Artemisia asiatica<\/jats:italic> Nakai. Recent research reports have proved the inhibitory function of Eupatilin in many diseases. MicroRNAs (miRNAs) are 21\u201323 nucleotide\u2010long, single\u2010stranded, noncoding RNA molecules generated endogenously, and many studies have indicated that miRNAs are involved in the development of osteoporosis. This study explored the role and potential mechanism of Eupatilin underlying PMOP. First, rats were given intragastric administration of Eupatilin every day and subcutaneous injections of oligonucleotides or plasmids that interfered with miR\u2010211\u20105p or janus kinase 2 (JAK2) once a week. After 4\u2009weeks, the PMOP rat model was established. Then, serum alkaline phosphatase, calcium, and phosphorus levels, as well as femur bone mineral density and biomechanical parameters, were detected. Hematoxylin\u2013eosin staining and Masson staining were applied for detecting the pathological condition of femur, and immunohistochemical staining was for detecting osteocalcin. MC3T3\u2010E1 cells were transfected with plasmid vectors interfering with miR\u2010211\u20105p or JAK2; and cell viability, lactate dehydrogenase cytotoxicity, and cell mineralization were subsequently examined. The relationship between miR\u2010211\u20105p and JAK2\/signal transducer and activator of transcription 3 (STAT3) pathway was analyzed. The targeting relation between miR\u2010211\u20105p and JAK2 was also verified. The experimental results revealed that Eupatilin improved the pathological conditions of PMOP rats by promoting the proliferation and mineralization of osteoblasts. MiR\u2010211\u20105p was down\u2010regulated and JAK2\/STAT3 was upregulated in PMOP rats. Upregulation of miR\u2010211\u20105p further improved the pathological conditions of PMOP rats based on Eupatilin treatment. MiR\u2010211\u20105p inhibited the JAK2\/STAT3 pathway. JAK2 offset the effects of elevated miR\u2010211\u20105p on PMOP rats. Overall, Eupatilin attenuates PMOP through elevating miR\u2010211\u20105p and repressing JAK2\/STAT3 pathway, which suggests the utility of Eupatilin as a potential drug for POMP treatment.<\/jats:p>","DOI":"10.1002\/tox.24069","type":"journal-article","created":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T06:39:24Z","timestamp":1703227164000},"page":"2218-2228","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Eupatilin ameliorates postmenopausal osteoporosis via elevating <scp>microRNA<\/scp>\u2010211\u20105p and repressing <scp>JAK2<\/scp>\/<scp>STAT3<\/scp> pathway"],"prefix":"10.1002","volume":"39","author":[{"given":"Liu","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Orthopedics Maternal and Child Health Hospital of Hubei Province  Wuhan Hubei 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