{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T14:44:36Z","timestamp":1778078676822,"version":"3.51.4"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T00:00:00Z","timestamp":1702857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Patrinia scabiosaefolia<\/jats:italic> Fisch (PS), a perennial herb belonging to the genus <jats:italic>Pinus<\/jats:italic> in the family Pinnacle Sauce, has been previously known for its analgesic, anti\u2010inflammatory, antibacterial, and antitumor properties. However, the specific mechanism behind its antileukemic effect remains unknown. This study focused on the cytotoxicity and potential modes of action of the dichloromethane extract from PS (DEPS) in acute myeloid leukemia (AML) cells. Our results demonstrated that DEPS reduced cell viability, arrested the cell cycle in the G2\/M phase, disrupted the mitochondrial membrane potential, increased reactive oxygen species (ROS) production, and upregulated the expression of Bax\/Bcl\u20102 and Cleaved caspase\u20103. However, the impact of DEPS on cell viability and the expression of apoptosis\u2010associated proteins was reversed upon pretreatment with the caspase\u20103 inhibitor (Z\u2010DEVD\u2010FMK) in HL\u201060 cells, which demonstrated that DEPS could induce apoptosis through the mitochondria\u2010associated apoptotic pathway. Interestingly, DEPS also influenced autophagy by upregulating the expression of LC3II\/I, P62, and Beclin\u20101 proteins, and the autophagy inhibition chloroquine(CQ) could attenuate the apoptotic effects of DEPS in HL\u201060 cells. Furthermore, SMART 2.0 analysis predicted that the main components present in DEPS were likely terpenoids. In conclusion, DEPS possibly exerts antileukemic effects by downregulating the PI3K\/AKT and ERK pathways, thereby promoting intracellular ROS production, activating the mitochondrial apoptotic pathway, and affecting autophagy, providing valuable insights for the potential future application of PS in the treatment of AML.<\/jats:p>","DOI":"10.1002\/tox.24090","type":"journal-article","created":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T14:56:30Z","timestamp":1702911390000},"page":"2123-2137","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Induction of apoptosis and autophagy by dichloromethane extract from <i>Patrinia scabiosaefolia<\/i> Fisch on acute myeloid leukemia cells"],"prefix":"10.1002","volume":"39","author":[{"given":"Kejing","family":"Li","sequence":"first","affiliation":[{"name":"The First Clinical Medical College Lanzhou University  Lanzhou Gansu 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Lanzhou Gansu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7489-4778","authenticated-orcid":false,"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"Central Laboratory, The First Hospital of Lanzhou University Lanzhou University  Lanzhou Gansu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yudong","family":"Pu","sequence":"additional","affiliation":[{"name":"Precision Medicine Center of the Songshan Lake (SSL) Central Hospital of Dongguan City  Dongguan Guangdong 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