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It is well\u2010established that house dust mites (HDMs) are common inducers of allergic responses in individuals, particularly children. In central Taiwan, our research team observed that over 80% of allergic children exhibited sensitization to various HDMs species. This investigation aims to bridge the gap between these observations and a better understanding of the early fundamental mechanisms for preventing allergic diseases. Specifically, our study delves into the impact of crude extracts of HDMs on human epithelial BEAS\u20102B cells. Our findings, based on RNA sequencing (RNA\u2010seq) analysis, shed light on how three major <jats:italic>Dermatophagoides<\/jats:italic> HDMs allergens activate a common Toll\u2010like receptor signaling pathway in human epithelial cells within a 4\u2010h treatment. During this process, the nuclear transcription factor NF\u2010\u03baB translocated into the cell nucleus within 30\u2009min of allergen stimulation, triggering the expression of pro\u2010inflammatory genes such as IL\u20106 and IL\u20108 over 4\u2009h. Additionally, when the cells were treated with specific <jats:italic>Dermatophagoides microceras (Der m)<\/jats:italic> allergens, it resulted in the upregulation of genes that regulate type 1 diabetes mellitus (T1DM) signaling pathways. This led to the mediation of IL\u201012A inflammation. Furthermore, there was an increase in gene sets associated with cilia function and the microtubule cytoskeleton in human epithelial cells after treatment with a combination of <jats:italic>Der m<\/jats:italic> allergens and Dexamethasone. Additionally, OMICs analysis was conducted to examine the effects of HDMs allergenic stimulation on human epidermal cells. We aimed to improve our understanding of the molecular mechanisms within cells and identify potential targets and natural products in the treatment of asthma caused by HDMs allergens.<\/jats:p>","DOI":"10.1002\/tox.24109","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T08:39:13Z","timestamp":1703147953000},"page":"2229-2239","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Multiplexed transcriptional profiling of <i>Dermatophagoides<\/i> house dust mites allergens in human epithelium cells"],"prefix":"10.1002","volume":"39","author":[{"given":"Chia\u2010Yang","family":"Lin","sequence":"first","affiliation":[{"name":"Institute of Medicine Chung Shan Medical University  Taichung Taiwan"},{"name":"Department of Family Medicine Chung Shan Medical University Hospital  Taichung 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