{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:42:56Z","timestamp":1791499376304,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173603"],"award-info":[{"award-number":["82173603"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cigarette smoking was known to accelerate the occurrence and development of bladder cancer by regulating RNA modification. However, the association between the combination of cigarette smoking and RNA modification\u2010related single nucleotide polymorphisms (RNAm\u2010SNPs) and bladder cancer risk remains unclear. In this study, 1681 participants, including 580 cases and 1101 controls, were recruited for genetic association analysis. In total, 1\u2009287\u2009990 RNAm\u2010SNPs involving nine RNA modifications (m\n                    <jats:sup>6<\/jats:sup>\n                    A, m\n                    <jats:sup>1<\/jats:sup>\n                    A, m\n                    <jats:sup>6<\/jats:sup>\n                    Am, 2'\u2010O\u2010Me, m\n                    <jats:sup>5<\/jats:sup>\n                    C, m\n                    <jats:sup>7<\/jats:sup>\n                    G, A\u2010to\u2010I, m\n                    <jats:sup>5<\/jats:sup>\n                    U, and pseudouridine modification) were obtained from the RMVar database. The interactive effect of cigarette smoking and RNAm\u2010SNPs on bladder cancer risk was assessed through joint analysis. The susceptibility analysis revealed that 89 RNAm\u2010SNPs involving m\n                    <jats:sup>6<\/jats:sup>\n                    A, m\n                    <jats:sup>1<\/jats:sup>\n                    A, and A\u2010to\u2010I modifications were associated with bladder cancer risk. Among them, m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010related rs2273058 in\n                    <jats:italic>CRNKL1<\/jats:italic>\n                    was associated with bladder cancer risk (odds ratios (OR)\u2009=\u20091.35,\n                    <jats:italic>p<\/jats:italic>\n                    <jats:sub>adj<\/jats:sub>\n                    \u2009=\u20091.78\u2009\u00d7\u200910\n                    <jats:sup>\u22124<\/jats:sup>\n                    ), and\n                    <jats:italic>CRNKL1<\/jats:italic>\n                    expression was increased in bladder cancer patients (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.035). Cigarette smoking combined with the A allele of rs2273058 increased bladder cancer risk compared with nonsmokers with the G allele of rs2273058 (OR\u2009=\u20092.40,\n                    <jats:italic>p<\/jats:italic>\n                    <jats:sub>adj<\/jats:sub>\n                    \u2009=\u20093.11\u2009\u00d7\u200910\n                    <jats:sup>\u22129<\/jats:sup>\n                    ). Mechanistically, the A allele of rs2273058 endowed\n                    <jats:italic>CRNKL1<\/jats:italic>\n                    with an additional m\n                    <jats:sup>6<\/jats:sup>\n                    A motif, facilitating recognition by m\n                    <jats:sup>6<\/jats:sup>\n                    A reader IGF2BP1, thereby promoting\n                    <jats:italic>CRNKL1<\/jats:italic>\n                    expression under cigarette smoking (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.142,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.017). Moreover, elevated\n                    <jats:italic>CRNKL1<\/jats:italic>\n                    expression may accelerate cell cycle and proliferation, thereby increasing bladder cancer risk. In summary, our study demonstrated that cigarette smoking combined with RNAm\u2010SNPs contributes to bladder cancer risk, which provides a potential target for bladder cancer prevention.\n                  <\/jats:p>","DOI":"10.1002\/tox.24138","type":"journal-article","created":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T06:26:07Z","timestamp":1706163967000},"page":"2782-2793","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cigarette smoking combined with genetic variation regulates the\n                    <scp>\n                      m\n                      <sup>6<\/sup>\n                      A\n                    <\/scp>\n                    methylation of\n                    <scp>\n                      <i>CRNKL1<\/i>\n                    <\/scp>\n                    and is associated with bladder cancer risk"],"prefix":"10.1002","volume":"39","author":[{"given":"Jialei","family":"Yang","sequence":"first","affiliation":[{"name":"Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research The Affiliated Cancer Hospital of Nanjing Medical University  Nanjing China"},{"name":"Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health Nanjing Medical University  Nanjing China"},{"name":"The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School Nanjing Medical University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Ji","sequence":"additional","affiliation":[{"name":"Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research The Affiliated Cancer Hospital of Nanjing Medical University  Nanjing China"},{"name":"Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Gao","sequence":"additional","affiliation":[{"name":"Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research The Affiliated Cancer Hospital of Nanjing Medical University  Nanjing China"},{"name":"Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health Nanjing Medical University  Nanjing China"},{"name":"Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajin","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research The Affiliated Cancer Hospital of Nanjing Medical University  Nanjing China"},{"name":"Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health Nanjing Medical University  Nanjing China"},{"name":"The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School Nanjing Medical University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mulong","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health Nanjing Medical University  Nanjing China"},{"name":"Department of Biostatistics, Center for Global Health, School of Public Health Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengdong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health Nanjing Medical University  Nanjing China"},{"name":"The Affiliated Taizhou People's Hospital of Nanjing Medical University  Taizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Urology Jiangsu Province Hospital of Traditional Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4996-958X","authenticated-orcid":false,"given":"Meilin","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research The Affiliated Cancer Hospital of Nanjing Medical University  Nanjing China"},{"name":"Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health Nanjing Medical University  Nanjing China"},{"name":"The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School Nanjing Medical University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-023-00744-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21763"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21338"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-021-01672-1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-2564"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)01169-7"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2023.03.029"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-022-03380-5"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar200057m"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1030"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232213851"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2021.07.022"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09712-x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.687"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI149807"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aay5525"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa811"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.985121"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202303473"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-022-02701-z"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2020.1722449"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.05.045"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau1646"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2023.131556"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2023.105584"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0361-y"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-22-2485"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1SC06832F"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2023.06.010"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00450-x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.01.006"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0168-5"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-018-0040-8"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0045-z"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-022-03173-w"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.13038"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.4161\/15592294.2014.969637"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02525-20"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2021.07.001"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2020.100728"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.artmed.2017.01.002"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.8261"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-1241.2004.00284.x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.29691"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.1004324"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3879"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJGM.S368573"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2016.138"}],"container-title":["Environmental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.24138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T08:46:56Z","timestamp":1762591616000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tox.24138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,25]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1002\/tox.24138"],"URL":"https:\/\/doi.org\/10.1002\/tox.24138","archive":["Portico"],"relation":{},"ISSN":["1520-4081","1522-7278"],"issn-type":[{"value":"1520-4081","type":"print"},{"value":"1522-7278","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,25]]},"assertion":[{"value":"2023-09-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}