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MiR\u20102110 was cloned and identified in Epstein\u2013Barr virus (EBV)\u2010positive NPC, but its role is unclear in NPC. In this study, we investigated the effect of miR\u20102110 on NPC metastasis and its related molecular basis. In addition, we also explored whether miR\u20102110 can be regulated by cinobufotalin (CB) and participate in the inhibition of CB on NPC metastasis. Bioinformatics, RT\u2010PCR, and in situ hybridization were used to observe the expression of miR\u20102110 in NPC tissues and cells. Scratch, Boyden, and tail vein metastasis model of nude mouse were used to detect the effect of miR\u20102110 on NPC metastasis. Western blot, Co\u2010IP, luciferase activity, colocalization of micro confocal and ubiquitination assays were used to identify the molecular mechanism of miR\u20102110 affecting NPC metastasis. Finally, miR\u20102110 induced by CB participates in CB\u2010stimulated inhibition of NPC metastasis was explored. The data showed that increased miR\u20102110 significantly suppresses NPC cell migration, invasion, and metastasis. Suppressing miR\u20102110 markedly restored NPC cell migration and invasion. Mechanistically, miR\u20102110 directly targeted FGFR1 and reduced its protein expression. Decreased FGFR1 attenuated its recruitment of NEDD4, which downregulated NEDD4\u2010induced phosphatase and tensin homolog (PTEN) ubiquitination and degradation and further increased PTEN protein stability, thereby inactivating PI3K\/AKT\u2010stimulated epithelial\u2013mesenchymal transition signaling and ultimately suppressing NPC metastasis. Interestingly, CB, a potential new inhibitory drug for NPC metastasis, significantly induced miR\u20102110 expression by suppressing PI3K\/AKT\/c\u2010Jun\u2010mediated transcription inhibition. Suppression of miR\u20102110 significantly restored cell migration and invasion in CB\u2010treated NPC cells. Finally, a clinical sample assay indicated that reduced miR\u20102110 was negatively correlated with NPC lymph node metastasis and positively related to NPC patient survival prognosis. In summary, miR\u20102110 is a metastatic suppressor involving in CB\u2010induced suppression of NPC metastasis.<\/jats:p>","DOI":"10.1002\/tox.24197","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T03:45:03Z","timestamp":1710301503000},"page":"3548-3562","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>MiR<\/scp>\n                    \u20102110 induced by chemically synthesized cinobufagin functions as a tumor\u2010metastatic suppressor via targeting\n                    <scp>FGFR1<\/scp>\n                    to reduce\n                    <scp>PTEN<\/scp>\n                    ubiquitination degradation in nasopharyngeal carcinoma"],"prefix":"10.1002","volume":"39","author":[{"given":"Shiyi","family":"Fang","sequence":"first","affiliation":[{"name":"Cancer Center, Integrated Hospital of Traditional Chinese Medicine Southern Medical University  Guangzhou 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