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It is well\u2010known that diquat\u2010induced oxidative stress is implicated in the intestinal barrier injury. Although some studies have shown that mitochondria are the primary target organelle of diquat, the underlying mechanism remains incompletely understood. Recently, mitochondria\u2010associated endoplasmic reticulum membranes (MAMs) have aroused increasing concerns among scholars, which participate in mitochondrial dynamics and signal transduction. In this study, we investigated whether MAMs involved in intestinal barrier injury and mitochondrial dysfunction induced by diquat\u2010induced oxidative stress in piglets and porcine intestinal epithelial cells (IPEC\u2010J2 cells). The results showed that diquat induced growth restriction and impaired intestinal barrier. The mitochondrial reactive oxygen species (ROS) was increased and mitochondrial membrane potential was decreased following diquat exposure. The ultrastructure of mitochondria and MAMs was also disturbed. Meanwhile, diquat upregulated endoplasmic reticulum stress marker protein and activated PERK pathway. Furthermore, loosening MAMs alleviated intestinal barrier injury, decrease of antioxidant enzyme activity and mitochondrial dysfunction induced by diquat in IPEC\u2010J2 cells, while tightening MAMs exacerbated diquat\u2010induced mitochondrial dysfunction. These results suggested that MAMs may be associated with the intestinal barrier injury and mitochondrial dysfunction induced by diquat in the jejunum of piglets.<\/jats:p>","DOI":"10.1002\/tox.24232","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T10:40:01Z","timestamp":1712140801000},"page":"3906-3919","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Mitochondria\u2010associated endoplasmic reticulum membranes involve in oxidative stress\u2010induced intestinal barrier injury and mitochondrial dysfunction under diquat exposing"],"prefix":"10.1002","volume":"39","author":[{"given":"Feiyang","family":"Gou","sequence":"first","affiliation":[{"name":"College of Animal Sciences, Zhejiang University Key Laboratory of Molecular Animal Nutrition (Zhejiang University) Ministry of Education  Hangzhou 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