{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:31:09Z","timestamp":1790094669197,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T00:00:00Z","timestamp":1712534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Backgrounds<\/jats:title><jats:p>Non\u2010small cell lung carcinoma (NSCLC) is a common type of lung cancer. Prior investigations have elucidated the pivotal role of miR\u201029b\u20103p in restraining tumor growth and metastasis. Nonetheless, it remains to be determined whether miR\u201029b\u20103p can effectively suppress NSCLC progression and enhance the sensitivity of NSCLC cells to cisplatin. This investigation sought to determine the mechanism by which miR\u201029b\u20103p inhibited the advancement of NSCLC and mitigated resistance to cisplatin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We initially assessed miR\u201029b\u20103p and VEGF levels in NSCLC tissues and cell lines. Next, miR\u201029b\u20103p expression was elevated in NSCLC cell lines H1975 and A549 by overexpression plasmid transfection. Following this, a sequence of molecular biology experiments was conducted to evaluate the impact of miR\u201029b\u20103p on the biological behaviors of NSCLC cells and their resistance to cisplatin. Additionally, we predicted VEGF was a target gene of miR\u201029b\u20103p by bioinformatics analysis. We next employed western blot to evaluate the protein expression of Nrf2 and HO\u20101 in NSCLC cells. Finally, we elucidated the effects of VEGF and Nrf2\/HO\u20101pathway on NSCLC progression and cisplatin resistance by in vitro assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In comparison to paracancerous tissues and human normal lung epithelial cells, the expression of miR\u201029b\u20103p was notably reduced and VEGF expression was clearly elevated in NSCLC tissues and cells. Moreover, miR\u201029b\u20103p upregulated obviously suppressed the biological activities of NSCLC cells and increased their sensitivity to cisplatin. Furthermore, in NSCLC cells, miR\u201029b\u20103p bound to VEGF and negatively regulate its transcription. Additionally, miR\u201029b\u20103p overexpression also inhibited the Nrf2\/HO\u20101 signaling pathway. Finally, the overexpression of VEGF and the activation of the Nrf2\/HO\u20101 pathway reversed miR\u201029b\u20103p\u2010mediated inhibitory effect on biological behaviors of NSCLC cells and increased the cisplatin resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings indicate that miR\u201029b\u20103p impedes NSCLC cells' biological behaviors and augments their sensitivity to cisplatin by targeting VEGF to modulate the Nfr2\/HO\u20101 signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/tox.24253","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T09:30:11Z","timestamp":1712568611000},"page":"3956-3966","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>miR\u201029b\u20103p<\/scp> targetedly regulates <scp>VEGF<\/scp> to inhibit tumor progression and cisplatin resistance through <scp>Nrf2\/HO\u20101<\/scp> signaling pathway in non\u2010small cell lung cancer"],"prefix":"10.1002","volume":"39","author":[{"given":"Zhen","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery Cangzhou Central Hospital  Cangzhou Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingming","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Cangzhou Central Hospital  Cangzhou Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieting","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Geriatrics Cangzhou Central Hospital  Cangzhou Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guojie","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Cangzhou Central Hospital  Cangzhou Hebei 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