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This study aimed to explore the immune\u2010related genes, immune microenvironment infiltration, and prognosis of CESC, providing a theoretical basis for guiding clinical treatment. Initially, by mining four gene sets and immune\u2010related gene sets from public databases, 14 immune\u2010related genes associated with CESC were identified. Through univariate and multivariate COX regression analyses, as well as lasso regression analysis, four CESC\u2010independent prognostic genes were identified, and a prognostic model was constructed, dividing them into high and low\u2010risk groups. The correlation between these genes and immune cells and immune functions were explored through ssGSEA enrichment analysis, revealing a close association between the high\u2010risk group and processes such as angiogenesis and epithelial\u2013mesenchymal transition. Furthermore, using public databases and qRT\u2010PCR experiments, significant differences in CXCL8 expression between normal cervical cells and cervical cancer cells were discovered. Subsequently, a CXCL8 knockdown plasmid was constructed, and the efficiency of CXCL8 knockdown was validated in two CESC cell lines, MEG\u201001 and HCE\u20101. Through CCK\u20108, scratch, and Transwell assays, it was confirmed that CXCL8 knockdown could inhibit the proliferation, invasion, and migration abilities of CESC cells. Targeting CXCL8 holds promise for personalized therapy for CESC, providing a strong theoretical basis for achieving clinical translation.<\/jats:p>","DOI":"10.1002\/tox.24283","type":"journal-article","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T12:45:21Z","timestamp":1712753121000},"page":"902-911","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The immune landscape and prognostic analysis of <scp>CXCL8<\/scp> immune\u2010related genes in cervical squamous cell carcinoma"],"prefix":"10.1002","volume":"40","author":[{"given":"Xiaoqian","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gynaecology and Obstetrics The Affiliated Suzhou Hospital of Nanjing Medical University  Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics The 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