{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T18:09:42Z","timestamp":1788890982993,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004391","name":"China Medical University Hospital","doi-asserted-by":"publisher","award":["DMR\u2010111\u2010107"],"award-info":[{"award-number":["DMR\u2010111\u2010107"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004391","name":"China Medical University Hospital","doi-asserted-by":"publisher","award":["DMR\u2010112\u2010089"],"award-info":[{"award-number":["DMR\u2010112\u2010089"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004391","name":"China Medical University Hospital","doi-asserted-by":"publisher","award":["DMR\u2010113\u2010072"],"award-info":[{"award-number":["DMR\u2010113\u2010072"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004391","name":"China Medical University Hospital","doi-asserted-by":"publisher","award":["DMR\u2010112\u2010088"],"award-info":[{"award-number":["DMR\u2010112\u2010088"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Osteoarthritis (OA) is a degenerative joint disease primarily affecting the elderly. It is characterized by the progressive decline of joint cartilage and alterations in the underlying bone. Several probiotic strains have exhibited immunomodulatory and anti\u2010inflammatory properties. Here, we examined the functions of live and dead\n                    <jats:italic>Clostridium butyricum<\/jats:italic>\n                    GKB7 (GKB7\u2010L and GKB7\u2010D) in a preclinical anterior cruciate ligament transection (ACLT)\u2010enhanced OA procedure. Oral administration of GKB7\u2010L and GKB7\u2010D ameliorated ACLT\u2010induced bone pain as assessed by weight\u2010bearing behavioral testing but did not affect body weight. Micro\u2010computed tomography (CT) results showed that GKB7\u2010L and GKB7\u2010D diminished ACLT\u2010induced bone destruction and loss. GKB7\u2010L and GKB7\u2010D\u2010enriched therapies also reduced ACLT\u2010induced production of the pro\u2010inflammatory cytokines interleukin (IL)\u20101\u03b2 and tumor necrosis factor (TNF)\u2010\u03b1, as well as the chondrolytic factor matrix metalloproteinase (MMP)\u20103, leading to inhibition of aggrecan and collagen type II degradation and thereby blocking cartilage breakdown. We therefore suggest that oral supplementation with GKB7\u2010L or GKB7\u2010D can be beneficial in the prevention and treatment of OA.\n                  <\/jats:p>","DOI":"10.1002\/tox.24367","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T09:09:53Z","timestamp":1719306593000},"page":"4927-4935","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Live and Dead\n                    <i>Clostridium butyricum<\/i>\n                    <scp>GKB7<\/scp>\n                    Diminish Osteoarthritis Pain and Progression in Preclinical Animal Model"],"prefix":"10.1002","volume":"39","author":[{"given":"Li\u2010Chai","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pharmacy Tajen University  Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen\u2010You","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Medicine, China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You\u2010Shan","family":"Tsai","sequence":"additional","affiliation":[{"name":"Biotech Research Institute, Grape King Bio Ltd.  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