{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T01:49:13Z","timestamp":1769824153538,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["305799\/2019\u20103"],"award-info":[{"award-number":["305799\/2019\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005667","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Inova\u00e7\u00e3o do Estado de Santa Catarina","doi-asserted-by":"publisher","award":["15\/2023"],"award-info":[{"award-number":["15\/2023"]}],"id":[{"id":"10.13039\/501100005667","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Pyriproxyfen (PPF) is an insecticide used in agriculture, which is approved for use in drinking water tanks for human consumption. However, some studies indicate that it may act as an endocrine disruptor and affect nontarget organisms. This study aimed to evaluate the effects of PPF on reproduction and general health status in female mice exposed from pre\u2010puberty to adulthood. In the first experiment, females were treated by gavage from postnatal day (PND) 23 to (PND) 75 and were distributed into three experimental groups: control (vehicle), PPF 0.1\u2009mg\/kg, and PPF 1\u2009mg\/kg. Female mice were assessed for the age of puberty onset, body mass, water and food consumption, and the estrous cycle. On PDN 75, a subgroup was euthanized, when vital and reproductive organs were collected and weighed. The thyroid, ovary, and uterus were evaluated for histomorphometry. The other subgroup was assessed in relation to reproductive performance and fetal parameters. In a second experiment, the uterotrophic assay was performed with juvenile females (PND 18) using doses of 0.01, 0.1, or 1\u2009mg\/kg of PPF. PPF treatment reduced thyroid mass and increased liver mass. Furthermore, there was an increase in ovarian interstitial tissue and, in the uterus, a decrease in the thickness of the endometrial stroma with reduced content of collagen fibers. There was also a reduction of 30% in pregnancy rate in the treated groups and an increase in the frequency of fetal death. This study suggests that, based on this experimental model, the insecticide may pose a reproductive risk for females chronically exposed to the substance from the pre\u2010pubertal period until adulthood. These results raise concerns about prolonged exposure of women to the same compound.<\/jats:p>","DOI":"10.1002\/tox.24374","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T08:05:26Z","timestamp":1721635526000},"page":"5019-5038","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Female Mice Exposed to Pyriproxyfen Since Prepuberty Showed Reproductive Impairment During Sexual Maturity and Increased Fetal Death in Their Offspring"],"prefix":"10.1002","volume":"39","author":[{"given":"Alice Santos","family":"da Silva","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o e Toxicologia (Laretox) Centro de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"},{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Biologia Celular e Do Desenvolvimento Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tainara Fernandes","family":"de Mello","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o e Toxicologia (Laretox) Centro de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"},{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Biologia Celular e Do Desenvolvimento Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique Frederico Enz","family":"Fag\u00e1","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o e Toxicologia (Laretox) Centro de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennyfer Karen","family":"Knorst","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o e Toxicologia (Laretox) Centro de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1tima Regina Mena Barreto","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Centro de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9437-6310","authenticated-orcid":false,"given":"Gabriel Adan Ara\u00fajo","family":"Leite","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o e Toxicologia (Laretox) Centro de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"},{"name":"Departamento de Biologia Celular Embriologia e Gen\u00e9tica, Centro de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Santa Catarina  Florian\u00f3polis SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1584\/jpestics.R08\u201002"},{"key":"e_1_2_10_3_1","unstructured":"World Health Organization \u201cPyriproxyfen in drinking\u2010water: Use for vector control in drinkingwater sources and containers. Background document for preparation of WHO Guidelines for drinking\u2010water quality \u201dhttps:\/\/cdn.who.int\/media\/docs\/default\u2010source\/wash\u2010documents\/wash\u2010chemicals\/pyriproxyfen\u2010background.pdf."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005651"},{"key":"e_1_2_10_5_1","unstructured":"Minist\u00e9rio da Sa\u00fade \u201cMonitoramento dos casos de arboviroses urbanas causados por v\u00edrus transmitidos pelo mosquito Aedes (dengue chikungunya e zika) semanas epidemiol\u00f3gicas 1 a 27 \u201dhttps:\/\/www.gov.br\/saude\/pt\u2010br\/centrais\u2010de\u2010conteudo\/publicacoes\/boletins\/epidemiologicos\/edicoes\/2021\/boletim_epidemiologico_svs_26.pdf\/view."},{"key":"e_1_2_10_6_1","unstructured":"Minist\u00e9rio da Sa\u00fade \u201cMonitoramento dos casos de arboviroses urbanas causados por v\u00edrus transmitidos pelo mosquito Aedes (dengue chikungunya e zika) semanas epidemiol\u00f3gicas 1 a 20 \u201dhttps:\/\/www.gov.br\/saude\/pt\u2010br\/centrais\u2010de\u2010conteudo\/publicacoes\/boletins\/epidemiologicos\/edicoes\/2021\/boletim_epidemiologico_svs_20.pdf\/view."},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2016.09.009"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.22355"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_1652_17"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.139496"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010020\u201008656\u2010w"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.4425"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2021.117654"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07\u20109574LSF"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2016.08.061"},{"key":"e_1_2_10_16_1","volume-title":"Report of the Twentieth WHOPES Working Group Meeting","author":"World Health Organization Pesticide Evaluation Scheme"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781444318777"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1519\u201069842002000400008"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287394.2018.1504702"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/68.2.473"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2005.02.001"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623308315665"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2014.01.004"},{"key":"e_1_2_10_24_1","volume-title":"Anomalias Cong\u00eanitas: Estudos Experimentais","author":"Damasceno D. C.","year":"2008"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.4377"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.01109821"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2018.11.031"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/09603120500155765"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2020.105490"},{"key":"e_1_2_10_30_1","unstructured":"Organisation for Economic Co\u2010operation and Development \u201c440 OECD Guideline for the Testing of Chemicals Uterotrophic Bioassay in Rodents: A Short\u2010Term Screening Test for Oestrogenic Properties \u201dhttps:\/\/www.oecd\u2010ilibrary.org\/environment\/test\u2010no\u2010440\u2010uterotrophic\u2010bioassay\u2010in\u2010rodents_9789264067417\u2010en."},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.37.879"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.21756"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10030471"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2021.112515"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.2486\/indhealth.40.345"},{"key":"e_1_2_10_36_1","unstructured":"Environmental Protection Agency \u201cHealth Effects Test Guidelines: OPPTS 870.4100 Chronic Toxicity \u201dhttps:\/\/www.regulations.gov\/document\/EPA\u2010HQ\u2010OPPT\u20102009\u20100156\u20100019."},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2018.04.023"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618\u2010018\u20100912\u20102"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.4.6635"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy115"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2023.109632"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230252824699"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2019.121835"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod45.1.27"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00029"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2021.118729"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2017.11.006"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2020.110973"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719118783881"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2014.03.015"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735\u2010022\u201010106\u20105"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.116055"}],"container-title":["Environmental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.24374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T09:29:23Z","timestamp":1762594163000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tox.24374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,22]]},"references-count":51,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/tox.24374"],"URL":"https:\/\/doi.org\/10.1002\/tox.24374","archive":["Portico"],"relation":{},"ISSN":["1520-4081","1522-7278"],"issn-type":[{"value":"1520-4081","type":"print"},{"value":"1522-7278","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,22]]},"assertion":[{"value":"2023-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}