{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T10:54:27Z","timestamp":1766832867571,"version":"build-2065373602"},"reference-count":55,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008641","name":"Chung Shan Medical University","doi-asserted-by":"publisher","award":["CSMU\u2010INT\u2010111\u2010010"],"award-info":[{"award-number":["CSMU\u2010INT\u2010111\u2010010"]}],"id":[{"id":"10.13039\/501100008641","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In patients with chronic kidney disease, the uremic toxin indoxyl sulfate (IS) accelerates kidney damage and the progression of cardiovascular disease. IS may contribute to vascular diseases by inducing inflammation in endothelial cells. Luteolin has documented antioxidant and anti\u2010inflammatory properties. This study aimed to investigate the effect of luteolin on IS\u2010mediated reactive oxygen species (ROS) production and intercellular adhesion molecule (ICAM\u20101) and monocyte chemoattractant protein (MCP\u20101) expression in EA.hy926 cells and the possible mechanisms involved. IS significantly induced ROS production (by 6.03\u2010fold,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), ICAM\u20101 (by 2.19\u2010fold,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) and MCP\u20101 protein expression (by 2.18\u2010fold,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), and HL\u201060 cell adhesion (by 31%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), whereas, luteolin significantly decreased IS\u2010induced ROS production, ICAM\u20101 and MCP\u20101 protein expression, and HL\u201060 cell adhesion. Moreover, luteolin attenuated IS\u2010induced nuclear accumulation of p65 and c\u2010jun. Luteolin dose\u2010dependently increased heme oxygenase\u20101 (HO\u20101) expression and the maximum fold induction of HO\u20101 by luteolin was 3.68\u2010fold (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), whereas, HO\u20101 knockdown abolished the suppression of ICAM\u20101 and MCP\u20101 expression by luteolin. Luteolin may protect against IS\u2010induced vessel damage by inducing HO\u20101 expression in vascular endothelial cells, which suppresses nuclear factor kappa B (NF\u2010\u03baB) and activator protein 1 (AP\u20101) mediated ICAM\u20101 and MCP\u20101 expression.\n                  <\/jats:p>","DOI":"10.1002\/tox.24380","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T06:07:05Z","timestamp":1722924425000},"page":"5112-5123","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Luteolin Inhibits Indoxyl Sulfate\u2010Induced\n                    <scp>ICAM<\/scp>\n                    \u20101 and\n                    <scp>MCP<\/scp>\n                    \u20101 Expression by Inducing\n                    <scp>HO<\/scp>\n                    \u20101 Expression in\n                    <scp>EA<\/scp>\n                    .hy926 Human Endothelial Cells"],"prefix":"10.1002","volume":"39","author":[{"given":"Li\u2010Chien","family":"Chang","sequence":"first","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine Armed Forces Taichung General Hospital  Taichung Taiwan"},{"name":"National Defense Medical Center Graduate Institute of Medical Sciences  Taipei Taiwan"},{"name":"Department of Nutrition Chung Shan Medical University  Taichung Taiwan"},{"name":"Central Taiwan University of Science and Technology  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"En\u2010Ling","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Nutrition, College of Medical and Health Care Hung\u2010Kuang University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Chi","family":"Chuang","sequence":"additional","affiliation":[{"name":"Department of Nutrition Chung Shan Medical University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia\u2010Hsuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Nutrition Chung Shan Medical University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia\u2010Wen","family":"Kuo","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine Armed Forces Taichung General Hospital  Taichung Taiwan"},{"name":"National Defense Medical Center Graduate Institute of Medical Sciences  Taipei Taiwan"},{"name":"Central Taiwan University of Science and Technology  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chong\u2010Kuei","family":"Lii","sequence":"additional","affiliation":[{"name":"Department of Nutrition China Medical University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Chen","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Food Nutrition and Health Biotechnology Asia University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7611-5515","authenticated-orcid":false,"given":"Haw\u2010Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Nutrition China Medical University  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5667-7489","authenticated-orcid":false,"given":"Chien\u2010Chun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nutrition Chung Shan Medical University  Taichung Taiwan"},{"name":"Department of Nutrition Chung Shan Medical University Hospital  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/138955709787001712"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2015.09.002"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2024.107236"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/08923973.2021.1905659"},{"key":"e_1_2_10_6_1","article-title":"Antitumor Activity of Luteolin Against Ehrlich Solid Carcinoma in Rats via Blocking Wnt\/Beta\u2010Zatenin\/SMAD4 Pathway","volume":"15","author":"Aljohani H.","year":"2023","journal-title":"Cureus"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.12974"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2016.142"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-967122"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2014.11.008"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60595-4"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-019-01718-5"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.116.005022"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.5.C1442"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2006.02.006"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000299798"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2023.122209"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2014.06.024"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2019.04.035"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20153628"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2012.10.002"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2020.114152"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2015.1037961"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2019.110811"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.09.205"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.107598"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO01352G"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2018-0397"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2007.02540.x"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.01.011"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00143.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.03980609"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins12040229"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/eos.12580"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-023-03104-2"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.05340610"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12882-017-0457-1"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0118703"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00659.2009"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2018060609"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9080752"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.5551\/jat.23697"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11152306"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118807"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2012.01.013"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2012.11.012"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.12.016"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X22500707"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.2960"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000022241"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000242336.58387.1f"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3858\/emm.2009.41.3.020"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-020-05346-1"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X19500289"},{"key":"e_1_2_10_55_1","first-page":"4156","article-title":"Randomized Clinical Trial \"Olfactory Dysfunction After COVID\u201019: Olfactory Rehabilitation Therapy vs. Intervention Treatment With Palmitoylethanolamide and Luteolin\": Preliminary Results","volume":"11","author":"D'Ascanio L.","year":"2021","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15020462"}],"container-title":["Environmental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.24380","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T09:29:45Z","timestamp":1762594185000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tox.24380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,6]]},"references-count":55,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/tox.24380"],"URL":"https:\/\/doi.org\/10.1002\/tox.24380","archive":["Portico"],"relation":{},"ISSN":["1520-4081","1522-7278"],"issn-type":[{"type":"print","value":"1520-4081"},{"type":"electronic","value":"1522-7278"}],"subject":[],"published":{"date-parts":[[2024,8,6]]},"assertion":[{"value":"2024-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}