{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T13:33:23Z","timestamp":1785504803123,"version":"3.56.0"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2016YFD0200504"],"award-info":[{"award-number":["2016YFD0200504"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","award":["322QN230"],"award-info":[{"award-number":["322QN230"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Spiromesifen (SPM) is widely used for orchard mites and white fly control. The ecotoxicological data suggested that SPM is highly toxic to fish, but the information about its toxic effect on zebrafish is still obscure. In this study, adult zebrafish were exposed to SPM for 21\u2009days. The plasma sex steroid hormone levels reflected the ratio of 17\u03b2\u2010estradiol (E2) to testosterone (T) (E2\/T) was significantly increased at 0.50\u2009\u03bcg\/L of SPM in male fish (2.4\u2010fold, <jats:italic>p<\/jats:italic>\u2009=\u20090.049). Following 21\u2009days' post exposure, distinct pathological changes were noted in gonad, males were more sensitive than female, which showed the interstitial connective tissue hyperplasia and widener in testis at 15\u2009\u03bcg\/L of SPM. In male fish, the relative percentage of spermatozoa was 13% decreased at 30\u2009\u03bcg\/L of SPM (<jats:italic>p<\/jats:italic>\u2009=\u20090.041). Which suggest SPM potential role in disrupting male gonad development. qRT\u2010PCR results suggest that expression of follicle stimulating hormone receptor (<jats:italic>fshr<\/jats:italic>) was significantly down regulated in female zebrafish (0.29 fold of control, <jats:italic>p<\/jats:italic>\u2009=\u20090.010). Variable importance of projection (VIP) scores indicate the most important features separate in female and male. The different response of steroid level towards SPM between male and female zebrafish may due to the distinct regulation of key genes related in steroidogenesis and metabolism. This study for the first time connects the biochemical and histological to reveal the adverse effects of SPM on adult zebrafish in a sex dependent manner.<\/jats:p>","DOI":"10.1002\/tox.24438","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T12:34:58Z","timestamp":1732624498000},"page":"598-607","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Sex Difference in Histopathological and Steroidogenesis Metabolism of Zebrafish After Exposure to Spiromesifen"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1281-1463","authenticated-orcid":false,"given":"Jie","family":"Zhang","sequence":"first","affiliation":[{"name":"Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Sciences China Agricultural University  Beijing People's Republic of China"},{"name":"Key Laboratory of Green Prevention and Control of Tropical 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0728-1943","authenticated-orcid":false,"given":"Chengju","family":"Wang","sequence":"additional","affiliation":[{"name":"Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Sciences China Agricultural University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06296-x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2022.107321"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2014.11.014"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx8004949"},{"key":"e_1_2_11_6_1","unstructured":"Transparency \u201cSpiromesifen Market \u2013 Global Industry Analysis Size Share Growth Trends 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