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This study examines the antifibrotic potential of CV ethanolic root extract (CVERE) against thioacetamide (TAA)\u2010induced liver fibrosis in Wistar rats. TAA was administered via i.p., thrice weekly for 11\u2009weeks to induce liver fibrosis in rats. In separate groups, rats were administered with TAA and were concurrently treated with CVERE 125\u2009mg\/kg, CVERE 250\u2009mg\/kg, and silymarin (SIL) 100\u2009mg\/kg. Liver marker enzymes of hepatotoxicity, oxidative stress markers, proinflammatory marker gene expression (TNF\u2010\u03b1, NF\u2010\u03baB, COX, and ILs), fibrotic marker gene expression (collagen I and III), immune histochemical expression of fibrosis marker proteins, and histopathologic changes were analyzed. TAA administration led to a significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) increase in the serum level of hepatotoxic marker enzymes. The TAA\u2010treated group showed higher levels (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) of MDA and reduced activities of SOD and CAT in the liver. TAA administration increased CYP2E1 expression, proinflammatory, and fibrotic marker gene expressions in rat liver. The histopathology of the liver confirms TAA\u2010induced architectural distortion and fibrotic changes. CVERE and SIL simultaneous treatments significantly protected against TAA\u2010induced oxidative stress, inflammation, and liver fibrosis. In conclusion, CVERE inhibited TAA\u2010induced liver fibrosis through downregulation of TAA metabolic activation, redox imbalance, and inflammation through repression of the NF\u2010\u03baB pathway.<\/jats:p>","DOI":"10.1002\/tox.24465","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T16:59:31Z","timestamp":1734713971000},"page":"723-736","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Coleus vettiveroides<\/i> Root Extract Protects Against Thioacetamide\u2010Induced Chronic Liver Injury by Inhibiting <scp>NF<\/scp>\u2010<scp>\u03baB<\/scp> Signaling Pathway"],"prefix":"10.1002","volume":"40","author":[{"given":"Kadmad Abdul Hameed","family":"Mohamed Azar","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Hepatology and Molecular Medicine Lab Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS)  Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5068-2383","authenticated-orcid":false,"given":"Devaraj","family":"Ezhilarasan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hepatology and Molecular Medicine Lab Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS)  Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munusamy","family":"Karthick","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hepatology and Molecular Medicine Lab Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS)  Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karthik","family":"Shree Harini","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hepatology and Molecular Medicine Lab Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS)  Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkatesan","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry College of Pharmacy, King Khalid University  Abha Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-024-17948-6"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2023.08.029"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI176345"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hbpd.2022.10.003"},{"volume-title":"Chronic Liver Disease","year":"2023","author":"Sharma A.","key":"e_1_2_9_6_1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2024.05.003"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30582"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30915"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.574276"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9040875"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2011.02.005"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43152\u2010021\u201000030\u20103"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/bsr20160107"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221\u20101691(12)60524\u20103"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3923\/rjmp.2010.206.212"},{"key":"e_1_2_9_17_1","first-page":"636","article-title":"Coleus Vettiveroides K.C. 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