{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T22:30:30Z","timestamp":1785537030413,"version":"3.56.0"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T00:00:00Z","timestamp":1735171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>AMP\u2010activated protein kinase (AMPK) is the master regulator of cellular energy which gets activated during energy stress and restores tissue homeostasis. AMPK is widely expressed in the pancreas and is involved in protein synthesis. In cerulein\u2010induced acute pancreatitis (AP), diminished AMPK activity in the pancreatic tissue may be associated with pancreatic inflammation and oxidative stress. Our results demonstrated that berberine (BR) treatment produced significant decrease in plasma amylase and lipase levels and improved histopathological features in AP mice model. Myeloperoxidase (MPO) activity indicated that BR suppressed the infiltration of neutrophils in pancreas. BR treatment markedly decreased the levels of proinflammatory cytokines including interleukins (IL)\u20106, IL\u20101\u03b2, and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) via inhibition of nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) expression. In addition, BR activates the nuclear factor erythroid 2\u2013related factor 2 (Nrf2) signaling and inhibits cerulein\u2010induced oxidative\u2010nitrosative stress. Mechanistically, we found inhibition of AMPK activity in cerulein\u2010induced AP, while BR\u2010treated animals showed marked increase in the AMPK expression. Together, our study indicated that BR\u2010mediated AMPK activation in pancreatic tissues demonstrated attenuation of cerulein\u2010induced oxidative stress and inflammation. Based on our observations, further exploration of this promising natural product against AP and associated complications may lead to promising therapeutic options.<\/jats:p>","DOI":"10.1002\/tox.24468","type":"journal-article","created":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T12:51:03Z","timestamp":1735217463000},"page":"764-773","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Berberine Attenuates Cerulein\u2010Induced Acute Pancreatitis by Modulating Nrf2\/<scp>NOX2<\/scp> Signaling Pathway via <scp>AMPK<\/scp> Activation"],"prefix":"10.1002","volume":"40","author":[{"given":"Sapana P.","family":"Bansod","sequence":"first","affiliation":[{"name":"Department of Biological Sciences (Regulatory Toxicology) National Institute of Pharmaceutical Education and Research (NIPER)  Hyderabad Telangana India"},{"name":"Division of Oncology, Department of Internal Medicine, Barnes\u2010Jewish Hospital and the Alvin J. 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