{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:08:30Z","timestamp":1789542510549,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Cuproptosis is a newly identified form of cell death that relies on copper (Cu) ionophores to transport Cu into cancer cells. As a perennial herb, <jats:italic>Patrinia scabiosaefolia<\/jats:italic> Fisch (PS) has garnered significant attention owing to its analgesic, anti\u2010inflammatory, antibacterial, and antitumor properties. Previous research has shown that the extract from PS (DEPS) can inhibit the growth of leukemia cell lines. However, the specific mechanism of its anti\u2010leukemic effect has not been fully clarified. Therefore, this study was conducted to investigate the molecular mechanism of cuproptosis in the treatment of leukemia with DEPS. Our results demonstrated that DEPS up\u2010regulated SLC31A1 and down\u2010regulated ATP7B expression, which increased intracellular copper concentration, down\u2010regulated FDX1, influenced the lipoylation of DLAT and DLD, and subsequently increased the expression of the stress protein HSP70 and the expression of PDHA1, inducing copper death in K562 cells. In addition, we investigated the toxicity of DEPS in\u00a0vivo and demonstrated its low in\u00a0vivo toxicity and adequate in\u00a0vivo safety. In conclusion, our results suggest that DEPS may induce cuproptosis in cells, offering valuable insights for the future application of PS in leukemia treatment.<\/jats:p>","DOI":"10.1002\/tox.24516","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T19:56:36Z","timestamp":1743796596000},"page":"1193-1206","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Induction of Cuproptosis by Dichloromethane Extract From <i>Patrinia scabiosaefolia<\/i> Fisch on <scp>K562<\/scp> Cells"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5909-7191","authenticated-orcid":false,"given":"Yuan","family":"Lu","sequence":"first","affiliation":[{"name":"The First Clinical Medical College Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyuan","family":"Mi","sequence":"additional","affiliation":[{"name":"The First 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