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The general health status of the EE\n                    <jats:sub>2<\/jats:sub>\n                    \u2010exposed tench was disturbed based on the increase of somatic indices at high tested doses, and the development of anemia in all exposed individuals. Effective control of reactive oxygen species by the antioxidant defense system of the tench exposed to EE\n                    <jats:sub>2<\/jats:sub>\n                    should have occurred because the lipid peroxidation process was irrelevant. Histopathological study revealed the presence of regressive changes in the liver (vacuolar degeneration, and deposits of eosinophilic material), regressive (deposits of eosinophilic material), and progressive (hyperplasia of reticuloendothelial cells) changes in the spleens of exposed fish. The severity of some lesions was dose dependent. The identified injuries did not compromise the functions of these organs. Finally, a common pattern of correlation between parameters of oxidative stress and morphological changes was not detected in the current study.\n                  <\/jats:p>","DOI":"10.1002\/tox.24557","type":"journal-article","created":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T06:21:38Z","timestamp":1755325298000},"page":"49-67","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Use of Oxidative Stress and Histological Biomarkers of\n                    <i>T. tinca<\/i>\n                    as Indicators of 17\u2010Alpha\u2010Ethynylestradiol Exposure"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4737-5371","authenticated-orcid":false,"given":"Ana L.","family":"Oropesa","sequence":"first","affiliation":[{"name":"Toxicology Area, School of Veterinary Medicine, University of Extremadura  C\u00e1ceres Spain"},{"name":"BRILiC \u2010 Biotechnology Research Institute in Livestock &amp; 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