{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:19:07Z","timestamp":1788275947967,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T00:00:00Z","timestamp":1775952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T00:00:00Z","timestamp":1775952000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005285","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005285","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Toxicology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fluopyram is a next\u2010generation fungicide widely used in agriculture; however, its persistence in soils and mobility toward aquatic systems have raised concerns regarding potential ecotoxicological risks. This study evaluated the cytogenotoxic effects of a commercial fluopyram\u2010based pesticide formulation on\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Allium cepa<\/jats:italic>\n                    <\/jats:styled-content>\n                    root meristem cells. Bulbs were exposed to four concentrations based on agricultural application rates: three times the recommended field dose (3\u2009\u00d7\u2009FD; 5.00\u2009mg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), the field dose (FD; 1.67\u2009mg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), half the field dose (0.5\u2009\u00d7\u2009FD; 0.835\u2009mg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), and one\u2010tenth the field dose (0.1\u2009\u00d7\u2009FD; 0.17\u2009mg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). Endpoints assessed included lipid peroxidation, cell viability, cytogenetic alterations, and DNA damage. No evidence of oxidative stress was detected. Nevertheless, the fluopyram\u2010based formulation induced significant chromosomal aberrations and nuclear abnormalities at all tested concentrations, indicating pronounced genotoxic potential. The comet assay revealed a low frequency of DNA strand breaks, suggesting an aneugenic mode of action likely associated with mitotic spindle disruption rather than clastogenic damage. Mitotic analysis showed an accumulation of cells in prophase, reinforcing the interpretation of chromosome missegregation.\n                    <jats:italic>In silico<\/jats:italic>\n                    docking analyses further demonstrated strong interactions between fluopyram ions and tubulin, providing mechanistic support for spindle\u2010associated aneugenicity. Overall, these findings highlight the cytogenotoxic risks associated with fluopyram\u2010based pesticides and reinforce the importance of environmental monitoring to protect nontarget organisms.\n                  <\/jats:p>","DOI":"10.1002\/tox.70096","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T06:50:56Z","timestamp":1776063056000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cytogenotoxic Effects of Fluopyram in\n                    <i>Allium cepa<\/i>\n                    Root Cells Involving Microtubule Disruption"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8173-5110","authenticated-orcid":false,"given":"Carlos Filipe","family":"Camilo\u2010Cotrim","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia, Universidade Estadual de Goi\u00e1s Unidade Universit\u00e1ria de Porangatu  Porangatu Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4984-8182","authenticated-orcid":false,"given":"Natanael Alves","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Universidade Estadual de Goi\u00e1s  An\u00e1polis Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8811-4886","authenticated-orcid":false,"given":"Gabriela Gomes","family":"Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Universidade Estadual de Goi\u00e1s  An\u00e1polis Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2242-9623","authenticated-orcid":false,"given":"Igor Rodrigues","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Universidade Estadual de Goi\u00e1s  An\u00e1polis Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7608-689X","authenticated-orcid":false,"given":"Luciana Souza","family":"Ondei","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biogeografia e Ecologia Aqu\u00e1tica Universidade Estadual de Goi\u00e1s  An\u00e1polis\u2010Go Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6737-7548","authenticated-orcid":false,"given":"Elisa Fl\u00e1via Luiz Cardoso","family":"Bail\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Universidade Estadual de Goi\u00e1s  An\u00e1polis Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9410-9194","authenticated-orcid":false,"given":"Ant\u00f4nio S\u00e9rgio Nakao","family":"de Aguiar","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Novos Materiais Universidade Evang\u00e9lica de Goi\u00e1s  An\u00e1polis Goi\u00e1s Brazil"},{"name":"Grupo de Qu\u00edmica Te\u00f3rica e Estrutural de An\u00e1polis Universidade Estadual de Goi\u00e1s  An\u00e1polis Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9008-6087","authenticated-orcid":false,"given":"Marcelino","family":"Benvindo\u2010Souza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Universidade Estadual de Goi\u00e1s  An\u00e1polis Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2183-3944","authenticated-orcid":false,"given":"Leonardo Luiz","family":"Borges","sequence":"additional","affiliation":[{"name":"Grupo de Qu\u00edmica Te\u00f3rica e Estrutural de An\u00e1polis Universidade Estadual de Goi\u00e1s  An\u00e1polis Goi\u00e1s Brazil"},{"name":"Escola de Ci\u00eancias M\u00e9dicas e da Vida Pontif\u00edcia Universidade Cat\u00f3lica de Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3811-4794","authenticated-orcid":false,"given":"Virg\u00ednia","family":"Damin","sequence":"additional","affiliation":[{"name":"Herbil, Universidade Federal de Goi\u00e1s Escola de Agronomia \u2010 Campus Samambaia  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1764-1480","authenticated-orcid":false,"given":"Luciane Madureira","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Universidade Estadual de Goi\u00e1s  An\u00e1polis Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/B978-0-12-819304-4.00003-8","volume-title":"Natural Remedies for Pest, Disease and Weed Control","author":"Abubakar Y.","year":"2020"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/10807039.2015.1133242"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010018\u20101439\u2010y"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201091460\u20104"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1088\/1742\u20106596\/2603\/1\/012054"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.21168\/rega.v17e16"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44169\u2010021\u201000001\u20107"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.25177\/JESES.4.3.RA.504"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2021.131830"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2019.109947"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2014.09.011"},{"key":"e_1_2_11_13_1","volume-title":"\u201cPublic Release Summary on the Evaluation of the New Active Constituent Fluopyram in the Product Transform Insecticide,\u201d APVMA Product No. 63642","author":"Australian Pesticides and Veterinary Medicines Authority","year":"2015"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0224132"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010022\u201020695\u2010z"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00087114.2018.1503496"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201017838\u20100"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2018.12.117"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2019.113551"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2014.11.007"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010022\u201021865\u20109"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399\u20103054.2006.00696.x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2011.570462"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616\u2010016\u20100044\u20105"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2006.12.003"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2009.03.014"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2009.06.002"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2003.12.008"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2012.06.001"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2022.114614"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac305"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/ci049885e"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ddtec.2004.11.015"},{"key":"e_1_2_11_34_1","volume-title":"R: A Language and Environment for Statistical Computing, Version 4.3.3","author":"R Core Team","year":"2024"},{"key":"e_1_2_11_35_1","volume-title":"RStudio: Integrated Development Environment for R, Version 2024.09.1","author":"Posit Team","year":"2024"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi801136q"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.13217"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.148034"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eraa608"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744\u20107909.2012.01177.x"},{"key":"e_1_2_11_41_1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/978-3-319-93233-0_13","volume-title":"Advances in Plant Ecophysiology Techniques","author":"Gra\u00f1a E.","year":"2018"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2010.05.011"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2010.10.006"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2022.113612"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/5161017"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/35019108"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1593\/tlo.08109"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616\u2010014\u20109741\u20100"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383\u20105718(01)00327\u20108"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1556\/ABiol.56.2005.1\u20102.12"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2018.04.177"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10661\u2010018\u20106616\u20104"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1080\/00087114.2015.1109927"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jes.2015.03.034"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415\u201047572009000200026"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11270\u2010016\u20102809\u2010z"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2017.12.052"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010019\u201004636\u2010x"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010020\u201008553\u20102"}],"container-title":["Environmental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.70096","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/tox.70096","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tox.70096","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T06:51:05Z","timestamp":1776063065000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/tox.70096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,12]]},"references-count":58,"alternative-id":["10.1002\/tox.70096"],"URL":"https:\/\/doi.org\/10.1002\/tox.70096","archive":["Portico"],"relation":{},"ISSN":["1520-4081","1522-7278"],"issn-type":[{"value":"1520-4081","type":"print"},{"value":"1522-7278","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,12]]},"assertion":[{"value":"2025-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"tox.70096"}}