{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:20:51Z","timestamp":1788402051840,"version":"build-2803163510"},"reference-count":84,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,7,18]],"date-time":"2021-07-18T00:00:00Z","timestamp":1626566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Genome"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The stiff\u2010stalk heterotic group in Maize (\n                    <jats:italic>Zea mays<\/jats:italic>\n                    L.) is an important source of inbreds used in U.S. commercial hybrid production. Founder inbreds B14, B37, B73, and, to a lesser extent, B84, are found in the pedigrees of a majority of commercial seed parent inbred lines. We created high\u2010quality genome assemblies of B84 and four expired Plant Variety Protection (ex\u2010PVP) lines LH145 representing B14, NKH8431 of mixed descent, PHB47 representing B37, and PHJ40, which is a Pioneer Hi\u2010Bred International (PHI) early stiff\u2010stalk type. Sequence was generated using long\u2010read sequencing achieving highly contiguous assemblies of 2.13\u20132.18 Gbp with N50 scaffold lengths &gt;200 Mbp. Inbred\u2010specific gene annotations were generated using a core five\u2010tissue gene expression atlas, whereas transposable element (TE) annotation was conducted using de novo and homology\u2010directed methodologies. Compared with the reference inbred B73, synteny analyses revealed extensive collinearity across the five stiff\u2010stalk genomes, although unique components of the maize pangenome were detected. Comparison of this set of stiff\u2010stalk inbreds with the original Iowa Stiff Stalk Synthetic breeding population revealed that these inbreds represent only a proportion of variation in the original stiff\u2010stalk pool and there are highly conserved haplotypes in released public and ex\u2010Plant Variety Protection inbreds. Despite the reduction in variation from the original stiff\u2010stalk population, substantial genetic and genomic variation was identified supporting the potential for continued breeding success in this pool. The assemblies described here represent stiff\u2010stalk inbreds that have historical and commercial relevance and provide further insight into the emerging maize pangenome.\n                  <\/jats:p>","DOI":"10.1002\/tpg2.20114","type":"journal-article","created":{"date-parts":[[2021,7,18]],"date-time":"2021-07-18T12:10:27Z","timestamp":1626610227000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Genomic variation within the maize stiff\u2010stalk heterotic germplasm pool"],"prefix":"10.1002","volume":"14","author":[{"given":"Nolan","family":"Bornowski","sequence":"first","affiliation":[{"name":"Dep. of Plant Biology Michigan State Univ.  612 Wilson Road East Lansing MI 48824 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn J.","family":"Michel","sequence":"additional","affiliation":[{"name":"Dep. of Agronomy Univ. of Wisconsin \u2013 Madison  1575 Linden Drive Madison WI 53706 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