{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:49:36Z","timestamp":1790891376272,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Genome"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Rice (\n                    <jats:italic>Oryza sativa<\/jats:italic>\n                    L.) is an excellent source of starch, which is composed of amylopectin and amylose. Resistant starch (RS) is a starch product that is not easily digestible and absorbed in the stomach or small intestine and instead is passed on directly to the large intestine. Cereals high in RS may be beneficial to improve human health and reduce the risk of diet\u2010related chronic diseases. It has been reported through chemical mutagenesis and RNA interference studies that starch branching enzymes (SBEs) play a major role in contributing to higher levels of RS in cereal crops. In this study, we used multiplex clustered regularly interspaced short palindromic repeat (CRISPR)\u2013CRISPR associated protein 9 (Cas9) genome editing to simultaneously target all four\n                    <jats:italic>SBE<\/jats:italic>\n                    genes in rice using the endogenous transfer RNA (tRNA)\u2010processing system for expressing the single\u2010guide RNAs (sgRNAs) targeting these genes. The CRISPR\u2013Cas9 vector construct with four\n                    <jats:italic>SBE<\/jats:italic>\n                    gene sgRNAs was transformed into the U.S. rice cultivar Presidio using\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    \u2010mediated transformation. Knockout mutations were identified at all four\n                    <jats:italic>SBE<\/jats:italic>\n                    genes across eight transgene\u2010positive T\n                    <jats:sub>0<\/jats:sub>\n                    plants. Transgene\u2010free T\n                    <jats:sub>1<\/jats:sub>\n                    lines with different combinations of disrupted\n                    <jats:italic>SBE<\/jats:italic>\n                    genes were identified, with several SBE\u2010edited lines showing significantly increased RS content up to 15% higher than the wild\u2010type (WT) cultivar Presidio. Although further efforts are needed to fix all of the mutant alleles as homozygous, our study demonstrated the potential of multiplex genome editing to develop high\u2010RS lines.\n                  <\/jats:p>","DOI":"10.1002\/tpg2.20225","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T05:19:37Z","timestamp":1655443177000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Increasing the level of resistant starch in \u2018Presidio\u2019 rice through multiplex CRISPR\u2013Cas9 gene editing of\n                    <i>starch branching enzyme<\/i>\n                    genes"],"prefix":"10.1002","volume":"16","author":[{"given":"Sudip","family":"Biswas","sequence":"first","affiliation":[{"name":"Dep. of Soil and Crop Sciences Texas A&amp;M Univ.  College Station TX 77843 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oneida","family":"Ibarra","sequence":"additional","affiliation":[{"name":"Dep. of Soil and Crop Sciences Texas A&amp;M Univ.  College Station TX 77843 USA"},{"name":"Avance Biosciences Inc.  Houston TX 77040 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam","family":"Shaphek","sequence":"additional","affiliation":[{"name":"Dep. of Soil and Crop Sciences Texas A&amp;M Univ.  College Station TX 77843 USA"},{"name":"Dep. of Biochemistry and Biophysics Texas A&amp;M Univ.  College Station TX 77843 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Molina\u2010Risco","sequence":"additional","affiliation":[{"name":"Dep. of Soil and Crop Sciences Texas A&amp;M Univ.  College Station TX 77843 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayra","family":"Faion\u2010Molina","sequence":"additional","affiliation":[{"name":"Dep. of Soil and Crop Sciences Texas A&amp;M Univ.  College Station TX 77843 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Bellinatti\u2010Della Gracia","sequence":"additional","affiliation":[{"name":"Dep. of Soil and Crop Sciences Texas A&amp;M Univ.  College Station TX 77843 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1868-6867","authenticated-orcid":false,"given":"Michael J.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Dep. of Soil and Crop Sciences Texas A&amp;M Univ.  College Station TX 77843 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9481-304X","authenticated-orcid":false,"given":"Endang M.","family":"Septiningsih","sequence":"additional","affiliation":[{"name":"Dep. of Soil and Crop Sciences Texas A&amp;M Univ.  College Station TX 77843 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01150"},{"key":"e_1_2_9_3_1","first-page":"S1","article-title":"Resistant starch. Proceeding from the 2nd plenary meeting of EURESTA: European FLAIR concerted action no. 11 on physiological implications of the consumption of resistant starch in man. 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