{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T17:03:47Z","timestamp":1782493427171,"version":"3.54.5"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,5,19]],"date-time":"2024-05-19T00:00:00Z","timestamp":1716076800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,5,19]],"date-time":"2024-05-19T00:00:00Z","timestamp":1716076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["Hatch\u00a01023186"],"award-info":[{"award-number":["Hatch\u00a01023186"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Genome"],"published-print":{"date-parts":[[2024,6]]},"abstract":"Abstract<\/jats:title>\n \n Dwarfism is a useful trait in many crop plants because it contributes to improved lodging resistance and harvest index. The mutant allele\n \n dw\n 3<\/jats:sub>\n \u2010ref\n <\/jats:italic>\n (\n \n dwarf\n 3<\/jats:sub>\n \u2010reference\n <\/jats:italic>\n ) of sorghum [\n Sorghum bicolor<\/jats:italic>\n (L.)\n Moench<\/jats:italic>\n ] is characterized by an 882\u00a0bp tandem duplication in the fifth exon of the gene that is unstable and reverts to wild\u2010type at a frequency greater than 0.001 in many genetic backgrounds. The goal of this research was to identify stable alleles of\n \n dw\n 3<\/jats:sub>\n <\/jats:italic>\n (\n dwarf3<\/jats:italic>\n ) that could be backcrossed into elite parent lines to improve height stability of the crop. To discover new alleles of\n \n dw\n 3<\/jats:sub>\n <\/jats:italic>\n , a panel consisting mostly of sorghum conversion lines (SC\u2010lines) was screened by polymerase chain reaction for the 882\u00a0bp tandem duplication in the fifth exon of\n \n dw\n 3<\/jats:sub>\n \u2010ref\n <\/jats:italic>\n . Sanger sequencing was used to characterize the DNA sequence of this fragment in genotypes that did not contain the 882\u00a0bp tandem duplication. Sequence analysis identified three indel mutations, including an 82\u00a0bp deletion, a 6\u00a0bp duplication, and a 15\u00a0bp deletion in this region of the gene. Field trials of the donor genotypes with these new alleles indicated no wild\u2010type revertants of\n \n dw\n 3<\/jats:sub>\n \u2010sd3\n <\/jats:italic>\n (\n \n dwarf\n 3<\/jats:sub>\n \u2010stable dwarf\n <\/jats:italic>\n ),\n \n dw\n 3<\/jats:sub>\n \u2010sd4\n <\/jats:italic>\n , and\n \n dw\n 3<\/jats:sub>\n \u2010sd5\n <\/jats:italic>\n . These alleles were backcrossed into Tx430. Field trials of backcross progeny (BC\n 2<\/jats:sub>\n F\n 4<\/jats:sub>\n ) with the\n \n dw\n 3<\/jats:sub>\n \u2010sd3\n <\/jats:italic>\n ,\n \n dw\n 3<\/jats:sub>\n \u2010sd4\n <\/jats:italic>\n , and\n \n dw\n 3<\/jats:sub>\n \u2010sd5\n <\/jats:italic>\n alleles indicated no revertants. The plant height and flowering time characteristics of the backcross progeny were similar or slightly shorter and earlier than the recurrent parent. These findings demonstrate that\n \n dw\n 3<\/jats:sub>\n \u2010sd3\n <\/jats:italic>\n ,\n \n dw\n 3<\/jats:sub>\n \u2010sd4\n <\/jats:italic>\n , and\n \n dw\n 3<\/jats:sub>\n \u2010sd5\n <\/jats:italic>\n alleles will be useful in breeding for the stable dwarf trait.\n <\/jats:p>","DOI":"10.1002\/tpg2.20466","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T01:59:20Z","timestamp":1716170360000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Mutations in the\n dwarf3<\/i>\n gene confer height stability in sorghum"],"prefix":"10.1002","volume":"17","author":[{"given":"Elisabeth","family":"Diatta\u2010Holgate","sequence":"first","affiliation":[{"name":"Agronomy Department Purdue University West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Bergsma","sequence":"additional","affiliation":[{"name":"Agronomy Department Purdue University West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5322-6519","authenticated-orcid":false,"given":"Mitchell R.","family":"Tuinstra","sequence":"additional","affiliation":[{"name":"Agronomy Department Purdue University West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.384"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci2011.12.0631"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci2007.02.0080"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/160.2.697"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.14.4008"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr944"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2540.2001.00895.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168\u20109525(02)00009\u20104"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/280457"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02858895"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/35093585"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086072"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010350"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001220051566"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.10.10.1623"}],"container-title":["The Plant Genome"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tpg2.20466","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tpg2.20466","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T12:20:11Z","timestamp":1761481211000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/10.1002\/tpg2.20466"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,19]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1002\/tpg2.20466"],"URL":"https:\/\/doi.org\/10.1002\/tpg2.20466","archive":["Portico"],"relation":{},"ISSN":["1940-3372","1940-3372"],"issn-type":[{"value":"1940-3372","type":"print"},{"value":"1940-3372","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,19]]},"assertion":[{"value":"2023-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e20466"}}