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This study investigated the genetic basis of bacterial wilt resistance in blueberry using a genome\u2010wide association study (GWAS) across two populations comprising 401 advanced selections from the University of Florida Blueberry Breeding and Genomics Program. A high\u2010throughput screening assay was developed to evaluate southern highbush blueberry responses to bacterial wilt based on leaf wilting severity and stem necrosis. Capture sequencing identified 38,379 single\u2010nucleotide polymorphisms. Moderate narrow\u2010sense heritability estimates were observed for leaf severity (0.26) and stem necrosis (0.20), and GWAS identified five small\u2010effect quantitative trait loci on chromosomes 1, 2, 5, and 11, each explaining 4.0%\u20137.4% of the phenotypic variance. Candidate gene analysis revealed putative pentatricopeptide repeat (PPR), serine\/threonine protein kinase, and MYB\u2010related proteins for leaf severity, and\n                    <jats:italic>Mlo<\/jats:italic>\n                    genes and polysaccharide biosynthesis genes for stem necrosis. Genomic selection (GS) analyses demonstrated potential for improving bacterial wilt resistance, with the GS\n                    <jats:italic>de novo<\/jats:italic>\n                    GWAS approach achieving the highest predictive ability by leveraging two key markers on chromosomes 1 and 11. These results elucidate the genetic architecture of bacterial wilt resistance in blueberries and provide resources for molecular breeding strategies to enhance resistance and ensure sustainable production.\n                  <\/jats:p>","DOI":"10.1002\/tpg2.70283","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T03:32:15Z","timestamp":1787283135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic insights into bacterial wilt resistance using genomic prediction and association mapping in blueberry"],"prefix":"10.1002","volume":"19","author":[{"given":"Lushan","family":"Ghimire","sequence":"first","affiliation":[{"name":"Blueberry Breeding and Genomics Lab, Horticultural Sciences Department University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma","family":"Flor","sequence":"additional","affiliation":[{"name":"Plant Pathology Department University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Adunola","sequence":"additional","affiliation":[{"name":"Blueberry Breeding and Genomics Lab, Horticultural Sciences Department University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip F.","family":"Harmon","sequence":"additional","affiliation":[{"name":"Plant Pathology Department University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Enciso\u2010Rodriguez","sequence":"additional","affiliation":[{"name":"Blueberry Breeding and Genomics Lab, Horticultural Sciences Department University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4698-2738","authenticated-orcid":false,"given":"Juliana","family":"Benevenuto","sequence":"additional","affiliation":[{"name":"Blueberry Breeding and Genomics Lab, Horticultural Sciences Department University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9655-4838","authenticated-orcid":false,"given":"Luis Felipe V.","family":"Ferr\u00e3o","sequence":"additional","affiliation":[{"name":"Blueberry Breeding and Genomics Lab, Horticultural Sciences Department University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8973-9351","authenticated-orcid":false,"given":"Patricio R.","family":"Munoz","sequence":"additional","affiliation":[{"name":"Blueberry Breeding and Genomics Lab, Horticultural Sciences Department University of Florida  Gainesville Florida USA"},{"name":"Plant Breeding Graduate Program University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1270\/jsbbs.20027"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppj2.70010"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3835\/plantgenome2016.01.0009"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/csc2.20075"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01387"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-21-1-0030"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci2013.05.0315"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-021-02455-w"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01316-18"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.01565"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.py.02.090164.001223"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81912-1"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkae163"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants13040488"},{"key":"e_1_2_11_16_1","doi-asserted-by":"crossref","unstructured":"Chen T. &Guestrin C.(2016)..Xgboost: A scalable tree boosting system. 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