{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T04:55:18Z","timestamp":1790571318871,"version":"4.1.0"},"reference-count":101,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Genome"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>DnaJ proteins (Hsp40s) are essential components of the cellular proteostasis network, functioning as molecular co\u2010chaperones that regulate protein folding, stability, and stress\u2010responsive homeostasis in plants. Beyond their classical role as Hsp70 partners, accumulating evidence demonstrates that DnaJ proteins participate in diverse biological processes by integrating proteostasis with hormonal, developmental, and defense signaling pathways. This review synthesizes recent advances in the structural diversity, evolutionary distribution, and functional specialization of DnaJ proteins across model plants and crops, including Arabidopsis, rice, maize, tomato, soybean, grapevine, citrus, cucumber, and other economically important species. We summarize mechanistic evidence demonstrating their involvement in abiotic stress tolerance, including heat, drought, salinity, osmotic, oxidative, and cold stress, through regulation of protein quality control, reactive oxygen species detoxification, ion homeostasis, abscisic acid and melatonin signaling, and transcriptional stress networks. Emerging studies further reveal their roles in biotic stress responses by modulating immune signaling, pathogen resistance, and host\u2013microbe interactions, while additional evidence links DnaJ proteins to reproductive development, root architecture, chloroplast stability, fruit ripening, and postharvest quality maintenance. Advances in genome\u2010wide identification, transcriptomic profiling, gene editing, transgenic validation, and functional genomics have substantially expanded understanding of DnaJ\u2010mediated molecular networks and their contributions to climate resilience. By integrating mechanistic biology with translational breeding strategies, this review highlights DnaJ proteins as promising molecular targets for crop improvement and climate\u2010smart agriculture aimed at increasing productivity, stress tolerance, and postharvest performance under changing environmental conditions.<\/jats:p>","DOI":"10.1002\/tpg2.70291","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T03:29:54Z","timestamp":1787282994000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Focus on DnaJ protein research from Arabidopsis to crop improvement"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9394-2774","authenticated-orcid":false,"given":"Muhammad","family":"Arif","sequence":"first","affiliation":[{"name":"College of Agriculture Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9554-4734","authenticated-orcid":false,"given":"Hafiz Mamoon","family":"Rehman","sequence":"additional","affiliation":[{"name":"Centre for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences The Chinese University of Hong Kong  Hong Kong China"},{"name":"Centre of Agricultural Biochemistry and Biotechnology (CABB) University of Agriculture Faisalabad  Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sehrish","family":"Bashir","sequence":"additional","affiliation":[{"name":"National Institute for Genomics and Advanced Biotechnology (NIGAB) National Agricultural Research Centre (NARC)  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noman","family":"Mahmood","sequence":"additional","affiliation":[{"name":"Mirpur University of Science and Technology (MUST) Mirpur  Azad Jammu &amp; Kashmir (AJK) Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atul Kumar","family":"Srivastava","sequence":"additional","affiliation":[{"name":"College of Agriculture Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yakov","family":"Kuzyakov","sequence":"additional","affiliation":[{"name":"Department of Soil Science of Temperate Ecosystems, Department of Agricultural Soil Science University of Goettingen  Gottingen Germany"},{"name":"Peoples Friendship University of Russia (RUDN University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moh Sajid","family":"Ansari","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6673-8740","authenticated-orcid":false,"given":"Hon\u2010Ming","family":"Lam","sequence":"additional","affiliation":[{"name":"Centre for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfang","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Management Science and Engineering Guizhou University of Finance and Economics  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"issue":"3","key":"e_1_2_18_2_1","first-page":"2758","article-title":"Plant heat shock proteins\u2010origin, evolution and mechanism of action for biotechnological implications in crop improvement","volume":"13","author":"Adhikari D.","year":"2024","journal-title":"International Journal of Innovative Research in Science, Engineering and Technology (IJIRSET)"},{"key":"e_1_2_18_3_1","doi-asserted-by":"crossref","DOI":"10.1590\/2175-7860202374020","article-title":"The DNAJ gene family in yerba mate (Ilex paraguariensis): Genome\u2010wide identification, structural characterization, orthology based classification and expression analysis","volume":"74","author":"Aguilera P. 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