{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T04:45:40Z","timestamp":1791866740848,"version":"4.3.4"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370707"],"award-info":[{"award-number":["32370707"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Genome"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    5\u2010Methylcytidine (m\n                    <jats:sup>5<\/jats:sup>\n                    C) modifications play a critical role in RNA metabolism. TRM4B (tRNA\u2010specific methytransferase 4B) is the only known component of the plant m\n                    <jats:sup>5<\/jats:sup>\n                    C machinery that mediates mRNA (messenger RNA) methylation. To date, no regulators governing site\u2010specific m\n                    <jats:sup>5<\/jats:sup>\n                    C modifications have been identified, apart from several degenerated sequence motifs. Drawing on relevant findings in animals, we investigated the involvement of AGO1 (Argonaute 1) in m\n                    <jats:sup>5<\/jats:sup>\n                    C regulation using an\n                    <jats:italic>ago1<\/jats:italic>\n                    mutant of\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    . m\n                    <jats:sup>5<\/jats:sup>\n                    C\u2010RIP\u2010seq (m\n                    <jats:sup>5<\/jats:sup>\n                    C RNA immunoprecipitation followed by deep sequencing) identified high\u2010confidence 5981 and 4559 m\n                    <jats:sup>5<\/jats:sup>\n                    C peaks in WT (wild type) and\n                    <jats:italic>ago1<\/jats:italic>\n                    , respectively. A substantial proportion of these peaks overlapped between WT and\n                    <jats:italic>ago1<\/jats:italic>\n                    , indicating their high gene specificity. Compared to WT,\n                    <jats:italic>ago1<\/jats:italic>\n                    exhibited 366 hyper\u2010methylated and 168 hypo\u2010methylated peaks. While both hyper\u2010 and hypo\u2010methylation could correlate with either up\u2010 or down\u2010regulated gene expression, actively transcribed genes with relatively complex structures were preferentially targeted by m\n                    <jats:sup>5<\/jats:sup>\n                    C modification. A significant fraction of m\n                    <jats:sup>5<\/jats:sup>\n                    C\u2010associated genes are functionally involved in mRNA surveillance and ribosome biogenesis. Intriguingly, over 60% of m\n                    <jats:sup>5<\/jats:sup>\n                    C peaks identified in WT were associated with the miRNA (microRNA) binding sites. Furthermore, AGO1 binding signals could be detected on the corresponding transcripts for &gt;40% of the m\n                    <jats:sup>5<\/jats:sup>\n                    C peaks based on the AGO1\u2010RIP\u2010seq experiment. Immunoprecipitation coupled to mass spectrometry validated the interaction between TRM4B and TRANSPORTIN1 (TRN1)\u2014a known AGO1\u2010interacting protein that is also implicated in the miRNA pathway. These findings support a model in which the miRNA\u2010guided AGO1 complex plays a critical role in mediating site\u2010specific m\n                    <jats:sup>5<\/jats:sup>\n                    C modifications.\n                  <\/jats:p>","DOI":"10.1002\/tpg2.70327","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:55:32Z","timestamp":1791863732000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The novel regulatory influence of Argonaute 1 on messenger RNA 5\u2010methylcytosine modification in\n                    <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1002","volume":"19","author":[{"given":"Zedi","family":"Feng","sequence":"first","affiliation":[{"name":"College of Life and Environmental Sciences Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu","family":"Yin","sequence":"additional","affiliation":[{"name":"School of Pharmacy Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifang","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiling","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0831-3086","authenticated-orcid":false,"given":"Xiaoxia","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Pharmacy Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4654-0622","authenticated-orcid":false,"given":"Yijun","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.04.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv416"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.12483"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870\u2010015\u20100580\u20108"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad1083"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2021.10.012"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030380405"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2017.09.013"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8367534"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.16.00384"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.16.00751"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.10.3971"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/2.10.1653"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00920"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014\u20104827(68)90403\u20105"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1120976"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.57.032905.105218"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2019.12.007"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859\u2010017\u20101808\u20104"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.85"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt171"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2014.06.008"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810241105"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010358"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.1.29"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq033"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyae027"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1754"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102012\u201013\u201010\u2010175"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbs040"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(76)90128\u20108"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2019.01.005"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010023\u201002872\u20106"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks144"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010023\u201000622\u2010x"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio848"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2008.04.007"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576\u2010020\u201000295\u20108"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2020.07.004"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201057969\u20102"}],"container-title":["The Plant Genome"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tpg2.70327","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/tpg2.70327","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/tpg2.70327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:55:33Z","timestamp":1791863733000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsess.onlinelibrary.wiley.com\/doi\/10.1002\/tpg2.70327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,12]]},"references-count":47,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["10.1002\/tpg2.70327"],"URL":"https:\/\/doi.org\/10.1002\/tpg2.70327","archive":["Portico"],"relation":{},"ISSN":["1940-3372","1940-3372"],"issn-type":[{"value":"1940-3372","type":"print"},{"value":"1940-3372","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,12]]},"assertion":[{"value":"2026-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70327"}}