{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T11:45:18Z","timestamp":1792410318621,"version":"4.4.0"},"reference-count":77,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acsess.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Genome"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Rice blast, caused by\n                    <jats:italic>Magnaporthe oryzae<\/jats:italic>\n                    , remains a major constraint to rice (\n                    <jats:italic>Oryza sativa<\/jats:italic>\n                    L.) production, and durable resistance will require broader use of genetic diversity beyond commonly studied elite cultivars. We present chromosome\u2010scale genome assemblies of two agronomically significant but genetically divergent rice cultivars: CT21375\u2010F4\u20104 (hereafter, CT21) and Moroberekan. Despite their genetic divergence, both cultivars show consistently strong resistance to blast under multiyear field conditions. We combined field phenotyping with genome\u2010wide protein\u2010to\u2010genome alignment, predicted proteome comparisons, nucleotide\u2010binding leucine\u2010rich repeat (NLR) annotation, and locus\u2010specific analyses of cloned blast resistance genes to examine resistance\u2010associated genomic variation in these cultivars. The cultivars contained similar numbers and overall subtype compositions of NLR loci, but differed markedly in their correspondence to characterized blast resistance genes. CT21 contained multiple exact or near\u2010exact matches to cloned blast resistance genes and alleles, whereas Moroberekan contained fewer exact matches and was characterized predominantly by high\u2010identity but divergent homologs, suggesting the presence of divergent alleles or resistance architectures that are not well represented by the cloned gene repertoire. Locus\u2010specific analyses further confirmed that high sequence similarity alone does not reliably predict resistance function, and revealed structural variation, including duplication within the\n                    <jats:italic>Pish<\/jats:italic>\n                    NLR cluster in CT21. These results demonstrate that comparable blast resistance phenotypes can occur in cultivars with distinct allelic and structural architectures at known resistance loci. The assemblies and comparative framework developed here provide resources for identifying resistance diversity that may be overlooked by reference gene or marker\u2010based analyses and for prioritizing candidate loci for functional validation and rice improvement.\n                  <\/jats:p>","DOI":"10.1002\/tpg2.70328","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T11:12:17Z","timestamp":1792408337000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative genome assemblies reveal distinct allelic and structural variation at blast resistance loci in rice CT21375\u2010F4\u20104 and Moroberekan"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7491-4468","authenticated-orcid":false,"given":"Federico","family":"Martin","sequence":"first","affiliation":[{"name":"Department of Agricultural Biology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9105-6266","authenticated-orcid":false,"given":"Jorge","family":"Duitama","sequence":"additional","affiliation":[{"name":"Systems and Computing Engineering Department Universidad de los Andes  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8061-4365","authenticated-orcid":false,"given":"Manuel","family":"Narvaez","sequence":"additional","affiliation":[{"name":"CIAT, Crops for Nutrition and Health  Cali Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3530-8251","authenticated-orcid":false,"given":"Alvaro L.","family":"Perez\u2010Quintero","sequence":"additional","affiliation":[{"name":"Plant Health Institute of Montpellier (PHIM) University of Montpellier, CIRAD, INRAE, Institut Agro, IRD  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5071-9935","authenticated-orcid":false,"given":"Constanza","family":"Quintero","sequence":"additional","affiliation":[{"name":"CIAT, Crops for Nutrition and Health  Cali Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8698-2679","authenticated-orcid":false,"given":"Kamal","family":"Neupane","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2686-474X","authenticated-orcid":false,"given":"Duvan S.","family":"Bonilla","sequence":"additional","affiliation":[{"name":"Systems and Computing Engineering Department Universidad de los Andes  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4369-5688","authenticated-orcid":false,"given":"Camila","family":"Rebolledo Cid","sequence":"additional","affiliation":[{"name":"CIRAD  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1104-6306","authenticated-orcid":false,"given":"M. Fernanda","family":"Alvarez","sequence":"additional","affiliation":[{"name":"CIAT, Crops for Nutrition and Health  Cali Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6622-7265","authenticated-orcid":false,"given":"Gloria M.","family":"Mosquera","sequence":"additional","affiliation":[{"name":"CIAT, Crops for Nutrition and Health  Cali Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7252-4397","authenticated-orcid":false,"given":"Jan E.","family":"Leach","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122\u2010026\u201005159\u2010z"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12284\u2010019\u20100311\u20100"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.108.095034"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.12.11.2033"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v080.i01"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201010\u2010421"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.107201"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13050891"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12284\u2010017\u20100159\u20100"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2011.03.010"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2018.10.007"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u2010313X.2006.02739.x"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010020\u201001056\u20105"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/978-0-306-48582-4_26","volume-title":"Rice blast: Interaction with rice and control","author":"Correa\u2010Victoria F. 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