{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:41:40Z","timestamp":1792096900890,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T00:00:00Z","timestamp":1637107200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Inserm","doi-asserted-by":"publisher","award":["U1071"],"award-info":[{"award-number":["U1071"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["UEG Journal"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>The identification of Crohn's disease (CD)\u2010associated adherent and invasive <jats:italic>Escherichia coli<\/jats:italic> (AIEC) is time\u2010consuming and requires ileal biopsies. We aimed to identify a faster and less invasive methods to detect ileal colonization by AIEC in CD patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CD patients requiring ileo\u2010colonoscopy were consecutively enrolled in this prospective multicenter study. Samples from saliva, serum, stools, and ileal biopsies of CD patients were collected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 102 CD patients, the prevalence of AIEC on ileal biopsies was 24.5%. The abundance and global invasive ability of ileal\u2010associated total <jats:italic>E. coli<\/jats:italic> were respectively ten\u2010fold (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0065) and two\u2010fold (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0007) higher in AIEC\u2010positive (vs. AIEC\u2010negative), while abundance of total <jats:italic>E. coli<\/jats:italic> in the feces was not correlated with AIEC status in the ileum. The best threshold of ileal total <jats:italic>E. coli<\/jats:italic> was 60 cfu\/biopsy to detect AIEC\u2010positive patients, with high negative predictive value (NPV) (94.1%[80.3\u201399.3]), while the global invasive ability (&gt;9000 internalized bacteria) was able to detect the presence of AIEC with high positive predictive value (80.0% [55.2\u2013100.0]). Overall, 78.1% of the AIEC\u00a0+\u00a0patients were colonized by two or less different AIEC strains. The level of serum anti\u2010total <jats:italic>E. coli<\/jats:italic> antibodies (AEcAb) was higher in AIEC\u2010positive patients (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.038) with a very high negative predictive value (96.6% [89.9\u2013100.0]) (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.038) for a cut\u2010off value\u00a0&gt;\u00a01.9\u00a0\u00d7\u00a010<jats:sup>\u22123<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>More than two thirds of AIEC\u2010positive CD patients were colonized by two or less AIEC strains. While stools samples are not accurate to screen AIEC status, the AEcAb level appears to be an attractive, rapid and easier biomarker to identify patients with Crohn's disease harboring AIEC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ueg2.12161","type":"journal-article","created":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T03:16:06Z","timestamp":1637205366000},"page":"1007-1018","source":"Crossref","is-referenced-by-count":31,"title":["Faster and less invasive tools to identify patients with ileal colonization by adherent\u2010invasive <i>E. coli<\/i> in Crohn's disease"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6347-409X","authenticated-orcid":false,"given":"Anthony","family":"Buisson","sequence":"first","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne\/Inserm U1071 USC\u2010INRAe 2018 Microbes, Intestin, Inflammation et Susceptibilit\u00e9 de l'H\u00f4te (M2iSH)  Clermont\u2010Ferrand France"},{"name":"Universit\u00e9 Clermont Auvergne Inserm 3iHP CHU Clermont\u2010Ferrand Service d'H\u00e9pato\u2010Gastro Ent\u00e9rologie  Clermont\u2010Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Vazeille","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne\/Inserm U1071 USC\u2010INRAe 2018 Microbes, Intestin, Inflammation et Susceptibilit\u00e9 de l'H\u00f4te (M2iSH)  Clermont\u2010Ferrand France"},{"name":"Universit\u00e9 Clermont Auvergne Inserm 3iHP CHU Clermont\u2010Ferrand Service d'H\u00e9pato\u2010Gastro Ent\u00e9rologie  Clermont\u2010Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2337-2902","authenticated-orcid":false,"given":"Mathurin","family":"Fumery","sequence":"additional","affiliation":[{"name":"Department of Hepatogastroenterology Amiens University Hospital, and PeriTox UMR\u2010I 01  Amiens 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