{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:30:41Z","timestamp":1791243041552,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T00:00:00Z","timestamp":1745020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019486","name":"Biocodex Microbiota Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019486","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["UEG Journal"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Faecalibacterium has recently garnered attention for its potential health implications. To better understand its role, we developed and assessed real\u2010time PCR assays for detecting and quantifying various Faecalibacterium species in human stool samples from both healthy individuals and Crohn's disease patients, either in flare or remission. The assays targeted the Microbial Anti\u2010inflammatory Molecule (MAM) genes, which encode MAM proteins. These assays demonstrated 100% species\u2010specificity using strains from six Faecalibacterium species: <jats:italic>Faecalibacterium prausnitzii<\/jats:italic>, <jats:italic>Faecalibacterium taiwanense<\/jats:italic>, <jats:italic>Faecalibacterium duncaniae<\/jats:italic>, <jats:italic>Faecalibacterium longum<\/jats:italic>, <jats:italic>Faecalibacterium hattori<\/jats:italic>, and <jats:italic>Faecalibacterium CNCM4541<\/jats:italic>. They also showed high sensitivity with detection limits of 10^5 bacteria per gram of sample. In healthy individuals, the different Faecalibacterium species varied in abundance. <jats:italic>F<\/jats:italic>. <jats:italic>taiwanense<\/jats:italic>, <jats:italic>F<\/jats:italic>. <jats:italic>duncaniae<\/jats:italic>, and <jats:italic>F<\/jats:italic>. <jats:italic>longum<\/jats:italic> were the most prevalent, around 10^10 bacteria\/g of stool. In contrast, <jats:italic>F<\/jats:italic>. <jats:italic>hattori<\/jats:italic> and CNCM4541 were less abundant, with 10^7 bacteria\/g. Despite its low abundance, <jats:italic>F<\/jats:italic>. <jats:italic>hattori<\/jats:italic> was present in all healthy subjects, while CNCM4541 was detected in only 50% of them. Notably, <jats:italic>F<\/jats:italic>. <jats:italic>taiwanense<\/jats:italic>, <jats:italic>F<\/jats:italic>. <jats:italic>duncaniae<\/jats:italic>, and <jats:italic>F<\/jats:italic>. <jats:italic>longum<\/jats:italic> were found in all healthy individuals. In Crohn's disease patients, both in flare and remission, a decrease in Faecalibacterium species was observed, with no recovery in remission. The most abundant species in Crohn's disease patients were <jats:italic>F<\/jats:italic>. <jats:italic>prausnitzii<\/jats:italic> and <jats:italic>F<\/jats:italic>. <jats:italic>duncaniae<\/jats:italic>, around 10^7 bacteria\/g, while <jats:italic>F<\/jats:italic>. <jats:italic>longum<\/jats:italic>, <jats:italic>F<\/jats:italic>. <jats:italic>hattori<\/jats:italic>, and <jats:italic>F<\/jats:italic>. <jats:italic>taiwanense<\/jats:italic> were present at lower levels (10^6 bacteria\/g), and CNCM4541 was no longer detected. Interestingly, <jats:italic>F<\/jats:italic>. <jats:italic>prausnitzii<\/jats:italic> showed a smaller decrease in abundance compared with other species. Moreover, <jats:italic>F<\/jats:italic>. <jats:italic>prausnitzii<\/jats:italic> was significantly more prevalent in patients in remission than in those in flare, suggesting that it may be more resistant to inflammation. These findings highlight the importance of accurately characterizing and quantifying Faecalibacterium species to better understand their role in health and disease.<\/jats:p>","DOI":"10.1002\/ueg2.70023","type":"journal-article","created":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T12:17:41Z","timestamp":1745065061000},"page":"1480-1490","source":"Crossref","is-referenced-by-count":13,"title":["<i>Faecalibacterium<\/i> Diversity in the Gut Microbiome of Crohn's Disease Patients"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7494-979X","authenticated-orcid":false,"given":"Paul","family":"Mclellan","sequence":"first","affiliation":[{"name":"Centre de Recherche Saint\u2010Antoine CRSA AP\u2010HP H\u00f4pital Saint Antoine Service de Gastroent\u00e9rologie Sorbonne Universit\u00e9 Inserm  Paris 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