{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:06:24Z","timestamp":1787317584553,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009336","name":"Bengt Ihres Foundation","doi-asserted-by":"publisher","award":["OLL\u2010709831"],"award-info":[{"award-number":["OLL\u2010709831"]}],"id":[{"id":"10.13039\/501100009336","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["UEG Journal"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Perinuclear\u2010antineutrophil cytoplasmic antibodies (pANCA) have been identified in familial ulcerative colitis (UC), but the mechanism underlying their expression remains elusive. We assessed the role of genetic predisposition, environmental factors and systemic subclinical inflammation in the development of pANCA in a twin cohort with UC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 48 twin pairs (Leuven, Belgium\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a04, Maastricht, The Netherlands\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a06 and \u00d6rebro, Sweden\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a038) with UC were included. Among these, 18 were monozygotic (3 concordant and 15 discordant for UC) and 30 were dizygotic (1 concordant and 29 discordant for UC). P\u2010ANCA was detected through standardised ELISA, an indirect immunofluorescence assay and DNase treatment. In addition to high sensitivity C\u2010reactive protein (hs\u2010CRP), 92 inflammatory protein markers were measured in serum by proximity extension assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      Perinuclear\u2010ANCA was present in 15\/52 (29%) of UC twins\n                      <jats:italic>vs<\/jats:italic>\n                      . 4\/44 (9%) healthy twin siblings (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.02). No agreement in the presence of pANCA or their levels was observed between twin siblings in monozygotic pairs discordant for UC [intraclass correlation coefficient (ICC)\u00a0=\u00a00.09] or dizygotic pairs (ICC\u00a0=\u00a0\u22120.20). Female sex was associated with an increased likelihood of pANCA (odds ratio, OR 5.25; 95% confidence interval, CI 1.36\u201320.30) and higher ANCA levels (ratio of geometric means 1.86; 95% CI 1.18\u20132.93). Active smoking was associated with lower concentrations of ANCA (ratio of geometric means 0.31; 95% CI 0.14\u20130.68) and potentially reduced the likelihood of pANCA (OR 0.20; 95% CI 0.03\u20131.34) in twins with UC but not in their healthy siblings. In healthy twin siblings, significant correlations between ANCA levels and hs\u2010CRP, CDCP1, IL17\u00a0A, CXCL9 and IL5 (correlation coefficients 0.36\u20130.41,\n                      <jats:italic>p<\/jats:italic>\n                      \u2010values &lt;\u00a00.05) were observed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Female sex and tobacco smoking outweighed genetics regarding the generation and levels of pANCA and ANCA antibodies. The correlations between ANCA levels and inflammatory markers in healthy twin siblings suggest that pANCA may result from subclinical inflammation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ueg2.70101","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T16:14:34Z","timestamp":1755792874000},"page":"1964-1973","source":"Crossref","is-referenced-by-count":1,"title":["Environmental and Inflammatory Factors Drive Perinuclear\u2010Antineutrophil Cytoplasmic Antibodies (pANCA) in Ulcerative Colitis: A European Twin Study"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1906-0746","authenticated-orcid":false,"given":"Daniel","family":"Bergemalm","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Faculty of Medicine and Health \u00d6rebro University  \u00d6rebro 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Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart","family":"Bodin","sequence":"additional","affiliation":[{"name":"Institute of Environmental Medicine Unit of Intervention and Implementation Research Karolinska Institute  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Carlson","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences Gastroenterology Research Group Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Halfvarson","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Faculty of Medicine and Health \u00d6rebro University  \u00d6rebro 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