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However, their widespread adoption has created important interpretive challenges for clinicians. Positive results may reflect colonization, prolonged shedding, or organisms of uncertain pathogenicity rather than active infection, and current panels do not provide antimicrobial susceptibility or quantitative information. Incomplete pathogen coverage and false\u2010positive detections may further complicate result interpretation, particularly in patients with mild or self\u2010limited disease. Inappropriate test ordering or overinterpretation of results may lead to unnecessary antimicrobial therapy, delayed evaluation of non\u2010infectious causes of diarrhea, and increased healthcare utilization. This review summarizes the key limitations of multiplex GI PCR panels and provides a practical framework for integrating molecular results with clinical presentation, disease severity, and patient risk factors to support informed management decisions in everyday clinical practice.<\/jats:p>","DOI":"10.1002\/ueg2.70272","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:55:46Z","timestamp":1785851746000},"source":"Crossref","is-referenced-by-count":1,"title":["Multiplex PCR to Diagnose Gastrointestinal Infections: Key Limitations in Interpretation"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8809-5168","authenticated-orcid":false,"given":"Giannoula S.","family":"Tansarli","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Washington School of Medicine  Seattle Washington 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