{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T18:52:41Z","timestamp":1783536761592,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T00:00:00Z","timestamp":1417651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fetal Medicine Foundation Belgium"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp;amp; Gyne"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To report clinical implementation of cell\u2010free <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> (cf<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>) analysis of maternal blood in screening for trisomies 21, 18 and 13 in twin pregnancies and examine variables that could influence the failure rate of the test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>cf<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> testing was performed in 515 twin pregnancies at 10\u201328 weeks' gestation. The failure rate of the test to provide results was compared with that in 1847 singleton pregnancies, and logistic regression analysis was used to determine which factors among maternal and pregnancy characteristics were significant predictors of test failure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Failure rate of the cf<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> test at first sampling was 1.7% in singletons and 5.6% in twins. Of those with a test result, the median fetal fraction in twins was 8.7% (range, 4.1\u201330.0%), which was lower than that in singletons (11.7% (range, 4.0\u201338.9%)). Multivariable regression analysis demonstrated that twin pregnancy, higher maternal weight and conception by <jats:italic>in\u2010vitro<\/jats:italic> fertilization provided significant independent prediction of test failure. Follow\u2010up was available in 351 (68.2%) of the twin pregnancies and comprised 334 with euploid fetuses, 12 discordant for trisomy 21 and five discordant for trisomy 18. In all 323 euploid cases with a result, the risk score for each trisomy was\u2009&lt;\u20091:10\u2009000. In 11 of the 12 cases with trisomy 21 and in the five with trisomy 18, the cf<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> test gave a high\u2010risk result, but in one case of trisomy 21, the score was\u2009&lt;\u20091:10\u2009000.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In twin pregnancies screening by cf<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> testing is feasible, but the failure rate is higher and detection rate may be lower than in singletons. Copyright \u00a9 2014 ISUOG. Published by John Wiley &amp; Sons Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/uog.14690","type":"journal-article","created":{"date-parts":[[2014,10,9]],"date-time":"2014-10-09T11:17:39Z","timestamp":1412853459000},"page":"61-66","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":106,"title":["Performance of screening for aneuploidies by cell\u2010free <scp>DNA<\/scp> analysis of maternal blood in twin pregnancies"],"prefix":"10.1002","volume":"45","author":[{"given":"E.","family":"Bevilacqua","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology University Hospital Brugmann  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. M.","family":"Gil","sequence":"additional","affiliation":[{"name":"Harris Birthright Research Centre for Fetal Medicine King's College Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. H.","family":"Nicolaides","sequence":"additional","affiliation":[{"name":"Harris Birthright Research Centre for Fetal Medicine King's College Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Ordo\u00f1ez","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Labco Diagnostic, Barcelona, Spain and Department of\u2009Biologia Cellular Universitat Autonoma de Barcelona  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Cirigliano","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Labco Diagnostic, Barcelona, Spain and Department of\u2009Biologia Cellular Universitat Autonoma de Barcelona  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Dierickx","sequence":"additional","affiliation":[{"name":"GENDIA (GENetic DIAgnostic Network)  Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. J.","family":"Willems","sequence":"additional","affiliation":[{"name":"GENDIA (GENetic DIAgnostic Network)  Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. C.","family":"Jani","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University Hospital Brugmann  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000358326"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2012.08.033"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4160"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1311037"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.14664"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.111.S1.1159"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003081-200403000-00016"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1375\/twin.10.2.406"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmt006"},{"key":"e_1_2_6_11_1","volume-title":"Amniocentesis and Chorionic Villus Sampling","author":"Royal College of Obstetricians and Gynaecologists (RCOG)","year":"2010"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2012.194068"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4132"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000355653"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000356495"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2012.01.030"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/GIM.0b013e3182368a0e"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000337373"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.12331"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2012.138"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000346806"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.5332"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep046"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000355527"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000323921"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.3892"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3109\/14767058.2012.733768"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4303"}],"container-title":["Ultrasound in Obstetrics &amp; Gynecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fuog.14690","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fuog.14690","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/uog.14690","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/uog.14690","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/uog.14690","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T23:52:41Z","timestamp":1694217161000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/uog.14690"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,4]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1002\/uog.14690"],"URL":"https:\/\/doi.org\/10.1002\/uog.14690","archive":["Portico"],"relation":{},"ISSN":["0960-7692","1469-0705"],"issn-type":[{"value":"0960-7692","type":"print"},{"value":"1469-0705","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,4]]},"assertion":[{"value":"2014-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-09-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}