{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T19:28:57Z","timestamp":1773430137458,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T00:00:00Z","timestamp":1541116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["PI13\/01449"],"award-info":[{"award-number":["PI13\/01449"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["RD12\/0026"],"award-info":[{"award-number":["RD12\/0026"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp; Gyne"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess potential differences in the expression of antiangiogenic and angiogenic factors and of genes associated with chronic hypoxia in cerebral tissue of euploid fetuses with congenital heart disease (CHD) <jats:italic>vs<\/jats:italic> those without.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cerebral tissue was obtained from 15 fetuses with CHD and 12 control fetuses that had undergone termination of pregnancy. Expression profiles of the antiangiogenic factor soluble fms\u2010like tyrosine kinase\u20101 (sFlt\u20101), the angiogenic vascular endothelial growth factor\u2010A (VEGF\u2010A) and placental growth factor (PlGF), and of genes associated with chronic hypoxia were determined by real\u2010time polymerase chain reaction in tissue from the frontal cortex and the basal ganglia of the fetuses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of sFlt\u20101 was 48% higher in the frontal cortex (<jats:italic>P<\/jats:italic>\u00a0=\u20090.0431) and 72% higher in the basal ganglia (<jats:italic>P<\/jats:italic>\u00a0=\u20090.0369) of CHD fetuses compared with controls. The expression of VEGF\u2010A was 60% higher (<jats:italic>P<\/jats:italic>\u00a0=\u20090.0432) and that of hypoxia\u2010inducible factor 2\u2010alpha was 98% higher (<jats:italic>P<\/jats:italic>\u00a0=\u20090.0456) in the basal ganglia of CHD fetuses compared with controls. No significant differences were observed between the two groups in the expression of PlGF and hypoxia\u2010inducible factor 1\u2010alpha.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>An overall dysregulation of angiogenesis with a net balance towards an antiangiogenic environment was observed in the cerebral tissue of fetuses with CHD, suggesting that these fetuses may have an intrinsic angiogenic impairment that could contribute to impaired brain perfusion and abnormal neurological development later in life. Copyright \u00a9 2017 ISUOG. Published by John Wiley &amp; Sons Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/uog.18977","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T12:19:10Z","timestamp":1512476350000},"page":"734-738","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Brain angiogenic gene expression in fetuses with congenital heart disease"],"prefix":"10.1002","volume":"52","author":[{"given":"O.","family":"S\u00e1nchez","sequence":"first","affiliation":[{"name":"Maternal and Child Health and Development Network (SAMID), RD16\/0022\/0015 Instituto de Salud Carlos III  Barcelona Spain"},{"name":"Biochemistry and Molecular Biology Research Centre for Nanomedicine Vall d'Hebron Research Institute (VHIR)  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Ruiz\u2010Romero","sequence":"additional","affiliation":[{"name":"Maternal\u2010Fetal Medicine Unit, Department of Obstetrics, Vall d'Hebron Research Institute (VHIR), SAMID Network Vall d'Hebron University Hospital  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Biology Research Centre for Nanomedicine Vall d'Hebron Research Institute (VHIR)  Barcelona Spain"},{"name":"Center for Biomedical Research on Rare Diseases (CIBERER) Instituto de Salud Carlos III  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Ferrer","sequence":"additional","affiliation":[{"name":"Pediatric Cardiology Unit, Department of Pediatrics Vall d'Hebron University Hospital  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Ribera","sequence":"additional","affiliation":[{"name":"Maternal\u2010Fetal Medicine Unit, Department of Obstetrics, Vall d'Hebron Research Institute (VHIR), SAMID Network Vall d'Hebron University Hospital  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Rodr\u00edguez\u2010Sureda","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Biology Research Centre for Nanomedicine Vall d'Hebron Research Institute (VHIR)  Barcelona Spain"},{"name":"Center for Biomedical Research on Rare Diseases (CIBERER) Instituto de Salud Carlos III  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Alijotas","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Vall d'Hebron University Hospital  Barcelona Spain"},{"name":"School of Medicine Universitat Aut\u00f2noma de Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Ar\u00e9valo","sequence":"additional","affiliation":[{"name":"Maternal\u2010Fetal Medicine Unit, Department of Obstetrics, Vall d'Hebron Research Institute (VHIR), SAMID Network Vall d'Hebron University Hospital  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Carreras","sequence":"additional","affiliation":[{"name":"Maternal\u2010Fetal Medicine Unit, Department of Obstetrics, Vall d'Hebron Research Institute (VHIR), SAMID Network Vall d'Hebron University Hospital  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Cabero","sequence":"additional","affiliation":[{"name":"Maternal\u2010Fetal Medicine Unit, Department of Obstetrics, Vall d'Hebron Research Institute (VHIR), SAMID Network Vall d'Hebron University Hospital  Barcelona Spain"},{"name":"School of Medicine Universitat Aut\u00f2noma de Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4048-6407","authenticated-orcid":false,"given":"E.","family":"Llurba","sequence":"additional","affiliation":[{"name":"Maternal\u2010Fetal Medicine Unit, Department of Obstetrics, Vall d'Hebron Research Institute (VHIR), SAMID Network Vall d'Hebron University Hospital  Barcelona Spain"},{"name":"School of Medicine Universitat Aut\u00f2noma de Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000437597.44550.5d"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000100782"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.064113"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2014.05.019"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2014.06.029"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2013.10.087"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa067393"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000032908.33237.b1"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e3181827bf4"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1047951107000248"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.13373"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.15970"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ppedcard.2010.06.011"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0b013e32834aa583"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.7474"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/14767058.2010.547961"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00246-002-0404-0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.12346"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/eht389"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH03-09-0582"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gav069"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24540"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2009-1959"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/dmcn.12242"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000439527"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.14919"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-1042"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0036-1584241"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(99)00125-X"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/61.4.339"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.07.015"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/359390"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2014-3825"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.18.3941"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.34.19761"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.5.2746"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.85.6.984"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2007.02.006"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.114.013051"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4762"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.12526"}],"container-title":["Ultrasound in Obstetrics &amp; Gynecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fuog.18977","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/uog.18977","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/uog.18977","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/uog.18977","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T05:52:21Z","timestamp":1694757141000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/uog.18977"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,2]]},"references-count":41,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1002\/uog.18977"],"URL":"https:\/\/doi.org\/10.1002\/uog.18977","archive":["Portico"],"relation":{},"ISSN":["0960-7692","1469-0705"],"issn-type":[{"value":"0960-7692","type":"print"},{"value":"1469-0705","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,2]]},"assertion":[{"value":"2017-05-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}