{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:54:12Z","timestamp":1791219252950,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T00:00:00Z","timestamp":1583107200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp; Gyne"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine the incidence of chromosomal abnormalities, submicroscopic chromosomal abnormalities and RASopathy\u2010disorder (RD) pathogenic variants in a cohort of pregnancies with nuchal translucency thickness (NT) \u2265\u20093.5\u2009mm, and to propose a clinical protocol for surveillance of this group of patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was a retrospective chart review of patients referred to The Prenatal Diagnosis and Medical Genetics Program at Mount Sinai Hospital between January 2013 and December 2015, due to NT \u2265\u20093.5\u2009mm, who underwent chorionic villus sampling or amniocentesis. Patients underwent extensive genetic counseling prior to invasive procedures and testing. Quantitative fluorescence polymerase chain reaction (QF\u2010PCR) was performed as the first\u2010line test for aneuploidy. If the result was negative, patients underwent karyotyping and\/or chromosomal microarray analysis (CMA), and if the findings were normal, they had testing for RD pathogenic variants, which included nine known genes. Patients also underwent detailed fetal ultrasound examinations and echocardiography, performed by expert operators.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 226 eligible patients were identified. In 116\/226 (51.3%) patients, QF\u2010PCR identified a chromosomal aneuploidy. The remaining 110\/226 (48.7%) patients had further genetic testing. Karyotyping\/CMA detected an abnormal\/pathogenic cytogenetic result in 9\/110 (8.2%) patients, as well as five variants of unknown significance (VOUS). RD testing yielded three pathogenic variants (3\/103), giving a detection rate of 2.9%, and one VOUS. The optimal NT cut\u2010off for RD screening was 7.9\u2009mm in this population. In 92\/110 (83.6%) patients, the genetic investigations were normal. Of these pregnancies, an early (14\u201316\u2009weeks' gestation) detailed fetal ultrasound examination identified a structural abnormality in 24 (26.1%), 15 (16.3%) had an abnormal detailed ultrasound examination at 18\u201322\u2009weeks' gestation and fetal echocardiography showed a cardiac abnormality in nine (9.8%). The birth outcome in the 83 pregnancies that had normal genetic investigations and known outcome included seven (8.4%) cases of termination of pregnancy, seven (8.4%) cases of intrauterine fetal death and 69 (83.1%) cases of live birth. Nine (9.8%) patients were lost to follow<jats:bold>\u2010<\/jats:bold>up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both CMA and molecular testing for RD are important investigations in pregnancies with NT \u2265\u20093.5\u2009mm. The use of genetic testing combined with fetal ultrasound examination provides valuable information that can influence pregnancy outcome, and provide recurrence risks, in this patient population. Copyright \u00a9 2019 ISUOG. Published by John Wiley &amp; Sons Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/uog.20352","type":"journal-article","created":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T09:25:37Z","timestamp":1558517137000},"page":"383-390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Microarray and RASopathy\u2010disorder testing in fetuses with increased nuchal translucency"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6711-8263","authenticated-orcid":false,"given":"P.","family":"Sinajon","sequence":"first","affiliation":[{"name":"The Prenatal Diagnosis and Medical Genetics Program, Department of Obstetrics and Gynecology Mount Sinai Hospital, University of Toronto  Toronto Ontario Canada"},{"name":"Division of Clinical and Metabolic Genetics The Hospital for Sick Children, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Chitayat","sequence":"additional","affiliation":[{"name":"The Prenatal Diagnosis and Medical Genetics Program, Department of Obstetrics and Gynecology Mount Sinai Hospital, University of Toronto  Toronto Ontario Canada"},{"name":"Division of Clinical and Metabolic Genetics The Hospital for Sick Children, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Roifman","sequence":"additional","affiliation":[{"name":"The Prenatal Diagnosis and Medical Genetics Program, Department of Obstetrics and Gynecology Mount Sinai Hospital, University of Toronto  Toronto Ontario Canada"},{"name":"Division of Clinical and Metabolic Genetics The Hospital for Sick Children, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Wasim","sequence":"additional","affiliation":[{"name":"University Health Network  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Carmona","sequence":"additional","affiliation":[{"name":"Medical Informatics Information Services, Joseph and Wolf Lebovic Health Complex, Mount Sinai Hospital, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Fetal Medicine Unit, Department of Obstetrics and Gynecology Mount Sinai Hospital, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Noor","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Medical Genetics, Department of Pathology and Laboratory Medicine Joseph and Wolf Lebovic Health Complex, Mount Sinai Hospital, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Kolomietz","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Medical Genetics, Department of Pathology and Laboratory Medicine Joseph and Wolf Lebovic Health Complex, Mount Sinai Hospital, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Chong","sequence":"additional","affiliation":[{"name":"The Prenatal Diagnosis and Medical Genetics Program, Department of Obstetrics and Gynecology Mount Sinai Hospital, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.304.6831.867"},{"key":"e_1_2_7_3_1","unstructured":"Perinatal Quality Foundation. 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