{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:32:10Z","timestamp":1791232330156,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2018YFC1004101"],"award-info":[{"award-number":["2018YFC1004101"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31900602"],"award-info":[{"award-number":["31900602"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31900664"],"award-info":[{"award-number":["31900664"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp; Gyne"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To examine the characteristics and distribution of possible severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) target cells in the human trophectoderm (TE) and placenta.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bioinformatics analysis was performed based on published single\u2010cell transcriptomic datasets of early TE and first\u2010 and second\u2010trimester human placentae. We conducted the transcriptomic analysis of 4198 early TE cells, 1260 first\u2010trimester placental cells and 189 extravillous trophoblast cells (EVTs) from 24\u2010week placentae (EVT_24W) using the SMART\u2010Seq2 method. In addition, to confirm the bioinformatic results, we performed immunohistochemical staining of three first\u2010trimester, three second\u2010trimester and three third\u2010trimester placentae from nine women recruited prospectively to this study. We evaluated the expression of the SARS\u2010CoV\u20102\u2010related molecules angiotensin\u2010converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Via bioinformatic analysis, we identified the existence of ACE2 and TMPRSS2 expression in human TE as well as in first\u2010 and second\u2010trimester placentae. In the human TE, 54.4% of TE1 cells, 9.0% of cytotrophoblasts (CTBs), 3.2% of EVTs and 29.5% of syncytiotrophoblasts (STBs) were ACE2\u2010positive. In addition, 90.7% of TE1 cells, 31.5% of CTBs, 22.1% of EVTs and 70.8% of STBs were TMPRSS2\u2010positive. In placental cells, 20.4% of CTBs, 44.1% of STBs, 3.4% of EVTs from 8\u2010week placentae (EVT_8W) and 63% of EVT_24W were ACE2\u2010positive, while 1.6% of CTBs, 26.5% of STBs, 1.9% of EVT_8W and 20.1% of EVT_24W were TMPRSS2\u2010positive. Pathway analysis revealed that EVT_24W cells that were positive for both ACE2 and TMPRSS2 (ACE2\u2009+\u2009TMPRSS2\u2010positive) were associated with morphogenesis of branching structure, extracellular matrix interaction, oxygen binding and antioxidant activity. The ACE2\u2009+\u2009TMPRSS2\u2010positive TE1 cells were correlated with an increased capacity for viral invasion, epithelial\u2010cell proliferation and cell adhesion. Expression of ACE2 and TMPRSS2 was observed on immunohistochemical staining in first\u2010, second\u2010 and third\u2010trimester placentae.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>ACE2\u2010 and TMPRSS2\u2010positive cells are present in the human TE and placenta in all three trimesters of pregnancy, which indicates the possibility that SARS\u2010CoV\u20102 could spread via the placenta and cause intrauterine fetal infection. \u00a9 2020 International Society of Ultrasound in Obstetrics and Gynecology<\/jats:p><\/jats:sec>","DOI":"10.1002\/uog.22186","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T13:20:06Z","timestamp":1598534406000},"page":"248-256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Single\u2010cell <scp>RNA<\/scp> expression profiling of SARS\u2010CoV\u20102\u2010related <scp>ACE2<\/scp> and <scp>TMPRSS2<\/scp> in human trophectoderm and placenta"],"prefix":"10.1002","volume":"57","author":[{"given":"D.","family":"Cui","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology Peking University First Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology Chinese Academy of Sciences  Beijing China"},{"name":"Institute for Stem Cell and Regeneration Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology Chinese Academy of Sciences  Beijing China"},{"name":"Institute for Stem Cell and Regeneration Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Ding","sequence":"additional","affiliation":[{"name":"Bill Lyons Informatics Centre, UCL Cancer Institute University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3944-4130","authenticated-orcid":false,"given":"L. C.","family":"Poon","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong, Prince of Wales Hospital  Shatin Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology Chinese Academy of Sciences  Beijing China"},{"name":"Institute for Stem Cell and Regeneration Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Peking University First Hospital  Beijing China"},{"name":"Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus  Beijing 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