{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:26:54Z","timestamp":1791581214481,"version":"4.3.4"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T00:00:00Z","timestamp":1615248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp;amp; Gyne"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the feasibility of amplification of the viral genome by polymerase chain reaction (PCR) analysis of trophoblast samples obtained by chorionic villus sampling (CVS) in cases of maternal primary infection (MPI) with cytomegalovirus (CMV) in early pregnancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was a prospective study carried out at the Department of Obstetrics and Fetal Medicine, Hopital Necker\u2010E.M., between October 2019 and October 2020. Following CMV serology screening in early pregnancy, CVS was offered to women at 11\u201314\u2009weeks' gestation after CMV\u2010MPI \u2264\u200910\u2009weeks. Array\u2010comparative genomic hybridization and amplification of the viral genome by PCR were performed on the trophoblasts obtained by CVS. All cases also underwent amniocentesis from 17\u2009weeks onwards and PCR was performed on the amniotic fluid. Secondary prevention with valacyclovir was initiated as soon as MPI was diagnosed, to decrease the risk of vertical transmission. We evaluated the diagnostic performance of CMV\u2010PCR of trophoblast obtained by CVS, using as the reference standard PCR of amniotic fluid obtained by amniocentesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CVS was performed in 37 pregnancies, at a median (range) gestational age of 12.7 (11.3\u201314.4)\u2009weeks. CMV\u2010PCR in chorionic villi was positive in three and negative in 34 cases. CMV\u2010PCR following amniocentesis, performed at a median (range) gestational age of 17.6 (16.7\u201329.9)\u2009weeks, was positive for the three cases which were positive following CVS and, of the 34 patients with a negative finding following CVS, amniocentesis was negative in 31 and positive in three. The sensitivity of CMV\u2010PCR analysis of trophoblast obtained by CVS for the diagnosis of CMV, using as the reference standard PCR analysis of amniotic fluid obtained by amniocentesis, was 50% (95%\u2009CI, 19\u201381%), specificity was 100% (95%\u2009CI, 89\u2013100%), positive predictive value was 100% (95%\u2009CI, 44\u2013100%) and negative predictive value was 91% (95%\u2009CI, 77\u201397%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Diagnosis of placental infection following MPI in early pregnancy can be achieved by PCR amplification of the CMV genome in chorionic villi. We propose that negative CMV\u2010PCR in the trophoblast after 12\u2009weeks could be used to exclude CMV\u2010related embryopathy leading to sequelae. However, this needs to be confirmed through long\u2010term follow\u2010up evaluation. These findings could help to establish CVS as the diagnostic test of choice following maternal serology screening in early pregnancy. \u00a9 2021 International Society of Ultrasound in Obstetrics and Gynecology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/uog.23608","type":"journal-article","created":{"date-parts":[[2021,2,6]],"date-time":"2021-02-06T01:04:12Z","timestamp":1612573452000},"page":"568-572","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["First\u2010trimester diagnosis of congenital cytomegalovirus infection after maternal primary infection in early pregnancy: feasibility study of viral genome amplification by <scp>PCR<\/scp> on chorionic villi obtained by <scp>CVS<\/scp>"],"prefix":"10.1002","volume":"57","author":[{"given":"V.","family":"Faure\u2010Bardon","sequence":"first","affiliation":[{"name":"EA 73\u201028 Universit\u00e9 de Paris  Paris France"},{"name":"AP\u2010HP, Department of Obstetrics and Fetal Medicine Hopital Necker\u2010E.M.  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Fourgeaud","sequence":"additional","affiliation":[{"name":"EA 73\u201028 Universit\u00e9 de Paris  Paris France"},{"name":"AP\u2010HP, Virology Laboratory Hopital Necker\u2010E.M.  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Guilleminot","sequence":"additional","affiliation":[{"name":"EA 73\u201028 Universit\u00e9 de Paris  Paris France"},{"name":"AP\u2010HP, Virology Laboratory Hopital Necker\u2010E.M.  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010F.","family":"Magny","sequence":"additional","affiliation":[{"name":"EA 73\u201028 Universit\u00e9 de Paris  Paris France"},{"name":"AP\u2010HP, Neonatal Intensive Care Unit Hopital Necker\u2010E.M  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. J.","family":"Salomon","sequence":"additional","affiliation":[{"name":"EA 73\u201028 Universit\u00e9 de Paris  Paris France"},{"name":"AP\u2010HP, Department of Obstetrics and Fetal Medicine Hopital Necker\u2010E.M.  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010P.","family":"Bernard","sequence":"additional","affiliation":[{"name":"EA 73\u201028 Universit\u00e9 de Paris  Paris France"},{"name":"AP\u2010HP, Department of Obstetrics and Fetal Medicine Hopital Necker\u2010E.M.  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Leruez\u2010Ville","sequence":"additional","affiliation":[{"name":"EA 73\u201028 Universit\u00e9 de Paris  Paris France"},{"name":"AP\u2010HP, Virology Laboratory Hopital Necker\u2010E.M.  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