{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T22:12:25Z","timestamp":1793052745853,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp;amp; Gyne"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The performance of non\u2010invasive prenatal screening using cell\u2010free DNA testing of maternal blood in twin pregnancy is underevaluated, while serum marker\u2010based strategies yield poor results. This study aimed to assess the performance of non\u2010invasive prenatal screening for trisomy 21 in twin pregnancy as a first\u2010tier test. Secondary objectives were to assess its failure rate and factors associated with failure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This retrospective cohort study included twin pregnancies in which non\u2010invasive prenatal screening using cell\u2010free DNA was performed as the primary screening strategy between May 2017 and October 2019. We used the NIPT VeriSeq\u00ae test for <jats:italic>in\u2010vitro<\/jats:italic> diagnosis and set a fetal fraction cut\u2010off of 4% for monochorionic pregnancies and 8% for dichorionic ones. Clinical data and pregnancy outcome were collected from physicians or midwives via a questionnaire or were retrieved directly on\u2010site. We calculated the performance of non\u2010invasive cell\u2010free DNA screening for trisomy 21, analyzed its failure rate and assessed potentially associated factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 1885 twin pregnancies with follow\u2010up, there were six (0.32%) confirmed cases of trisomy 21. The sensitivity of non\u2010invasive prenatal screening for trisomy 21 was 100% (95%\u2009CI, 54.1\u2013100%) and the false\u2010positive rate was 0.23% (95%\u2009CI, 0.06\u20130.59%). The primary failure rate was 4.6%, with 4.0% being due to insufficient fetal fraction. A successful result was obtained for 65.4% of women who underwent a new blood draw, reducing the overall failure rate to 2.8%. Maternal body mass index, gestational age at screening as well as chorionicity were significantly associated with the risk of failure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides further evidence of the high performance, at an extremely low false\u2010positive rate, of non\u2010invasive prenatal screening in twins as part of a primary screening strategy for trisomy 21. \u00a9 2023 The Authors. <jats:italic>Ultrasound in Obstetrics &amp; Gynecology<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/uog.26311","type":"journal-article","created":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T15:29:13Z","timestamp":1689866953000},"page":"807-814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Non\u2010invasive cell\u2010free <scp>DNA<\/scp> prenatal screening for trisomy 21 as part of primary screening strategy in twin pregnancy"],"prefix":"10.1002","volume":"63","author":[{"given":"N.","family":"Claudel","sequence":"first","affiliation":[{"name":"Maternit\u00e9 Port Royal, AP\u2010HP H\u00f4pital Cochin FHU Prema  Paris France"},{"name":"Universit\u00e9 Paris Cit\u00e9  Paris France"},{"name":"INSERM UMR_S1139  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Barrois","sequence":"additional","affiliation":[{"name":"Maternit\u00e9 Port Royal, AP\u2010HP H\u00f4pital Cochin FHU Prema  Paris France"},{"name":"Universit\u00e9 Paris Cit\u00e9  Paris France"},{"name":"INSERM UMR_S1139  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4921-0047","authenticated-orcid":false,"given":"A. 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