{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:50:19Z","timestamp":1791924619004,"version":"4.3.4"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,9,29]],"date-time":"2024-09-29T00:00:00Z","timestamp":1727568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp;amp; Gyne"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare the diagnostic performance of different manufacturers' immunoassays for the soluble fms\u2010like tyrosine kinase\u20101 (sFlt\u20101)\u2010to\u2010placental growth factor (PlGF) ratio with that of a point\u2010of\u2010care (PoC) test for glycosylated fibronectin (GlyFn) in women with suspected pre\u2010eclampsia (PE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was a prospective, single\u2010center, double\u2010blinded, non\u2010interventional study of East Asian women with a singleton pregnancy who presented with hypertension with or without clinical features of PE after 20\u2009weeks' gestation between January 2020 and March 2022. Maternal serum samples were collected at the time of presentation, and subsequent management followed the departmental protocol, based on gestational age, severity of hypertension, fetal condition and presence of severe PE features. Women diagnosed with PE at presentation were excluded. PE was diagnosed according to the 2018 International Society for the Study of Hypertension in Pregnancy classification. Levels of sFlt\u20101 and PlGF were measured using the Cobas e411 (Roche Diagnostics), BRAHMS KRYPTOR (ThermoFisher Scientific) and iMAGIN\u20091800 (Ningbo Aucheer) platforms. GlyFn levels were measured using the Lumella\u2122 GlyFn PoC test (DiabetOmics Inc.). The predictive performance of each test to rule out PE within 7\u2009days and rule in PE within 28\u2009days from the date of presentation was assessed. Based on the PROGNOSIS study, a  sFlt\u20101\/PlGF ratio of \u2264\u200938 on the Roche platform was used to predict the absence of PE within 7\u2009days. The sFlt\u20101\/PlGF ratio was classified as high or low using platform\u2010specific thresholds equivalent to a Roche sFlt\u20101\/PlGF ratio of 38, which were derived using Passing\u2013Bablok regression. GlyFn was categorized as high or low using two reported clinical management thresholds (263\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL and 510\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 236 women with suspected PE were included, of whom 70 (29.7%) were diagnosed with PE; 36 (51.4%) and 70 (100%) developed PE within 7\u2009days and 28\u2009days, respectively. Eighty\u2010eight (37.3%) women had a sFlt\u20101\/PlGF ratio of &gt;\u200938 on the Roche platform, 79 (33.5%) women had a sFlt\u20101\/PlGF ratio of &gt;\u200955 on the KRYPTOR platform and 96 (40.7%) women had a sFlt\u20101\/PlGF ratio of &gt;\u200940 on the iMAGIN\u20091800 platform. Furthermore, 62 (26.3%) and four (1.7%) women had a GlyFn level of &gt;\u2009263\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL and &gt;\u2009510\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL, respectively. The negative predictive value (NPV) of the sFlt\u20101\/PlGF ratio measured on the Roche, KRYPTOR and iMAGIN\u20091800 platforms to rule out PE within 7\u2009days after presentation was 83.3%, 82.0% and 82.9%, respectively, while that for GlyFn &gt;\u2009263\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL and &gt;\u2009510\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL was 82.6% and 70.4%, respectively. The corresponding positive predictive values (PPV) to rule in PE within 28\u2009days after presentation were 50.5%, 52.3% and 46.7%, respectively, for the sFlt\u20101\/PlGF ratio, and 35.4% and 50.0%, respectively, for GlyFn &gt;\u2009263\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL and &gt;\u2009510\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The predictive performance of different manufacturers' assays for the sFlt\u20101\/PlGF ratio to rule in and rule out PE were similar once standardized to a common threshold. Our findings suggest that the sFlt\u20101\/PlGF ratio and GlyFn using a cut\u2010off of 263\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL can both be utilized to rule out PE within 7\u2009days after assessment, with a moderate NPV. The PPV for ruling in PE within 28\u2009days remains poor. \u00a9 2024 The Author(s). <jats:italic>Ultrasound in Obstetrics &amp; Gynecology<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/uog.29107","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T06:56:54Z","timestamp":1727679414000},"page":"620-625","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Intermanufacturer assessment of diagnostic performance of angiogenic ratio <i>vs<\/i> glycosylated fibronectin in women with suspected pre\u2010eclampsia"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4586-6054","authenticated-orcid":false,"given":"I. Y. M.","family":"Wah","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3755-2701","authenticated-orcid":false,"given":"D. S.","family":"Sahota","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong SAR China"},{"name":"Shenzhen Research Institute The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2082-8476","authenticated-orcid":false,"given":"N. K. L.","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4565-1280","authenticated-orcid":false,"given":"N. M. W.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. J.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. S. L.","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2733-8939","authenticated-orcid":false,"given":"H. H. Y.","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3944-4130","authenticated-orcid":false,"given":"L. C.","family":"Poon","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology The Chinese University of Hong Kong  Hong Kong SAR China"},{"name":"Shenzhen Research Institute The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.12802"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.10803"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32335-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm7117a1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/hr.2016.126"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.119.12760"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1414838"},{"key":"e_1_2_8_9_1","unstructured":"National Institute for Health and Care Excellence.PlGF\u2010based testing to help diagnose suspected pre\u2010eclampsia (Triage PlGF test Eis\u2010Pr DELFIA Xpress PlGF 1\u20102\u20103 test and BRAHMS sFlt\u20101 Kryptor\/BRAHMS PlGF plus Kryptor PE ratio).2016."},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.113.003215"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2019.06.004"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008808"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2021.06.004"},{"issue":"464","key":"e_1_2_8_14_1","first-page":"e461","article-title":"Urinary congophilia in women with hypertensive disorders of pregnancy and preexisting proteinuria or hypertension","volume":"215","author":"McCarthy FP","year":"2016","journal-title":"Am J Obstet Gynecol"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(92)91663-U"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2023.03.003"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.119.12739"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.16323"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.27458"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2388"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2017.12.002"},{"issue":"3","key":"e_1_2_8_22_1","first-page":"45","article-title":"Soluble fms\u2010like tyrosine kinase\u20101 and placental growth factor in Chinese pregnant women during second and third trimesters","volume":"24","author":"Cheng YK","year":"2018","journal-title":"Hong Kong Med J"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12884-020-2809-2"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)33212-4"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.27451"}],"container-title":["Ultrasound in Obstetrics &amp; Gynecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/uog.29107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T16:31:42Z","timestamp":1739982702000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/uog.29107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,29]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/uog.29107"],"URL":"https:\/\/doi.org\/10.1002\/uog.29107","archive":["Portico"],"relation":{},"ISSN":["0960-7692","1469-0705"],"issn-type":[{"value":"0960-7692","type":"print"},{"value":"1469-0705","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,29]]},"assertion":[{"value":"2024-05-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}