{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:34:16Z","timestamp":1787236456293,"version":"build-2736575974"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T00:00:00Z","timestamp":1740960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp;amp; Gyne"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the presence and levels of cytomegalovirus (CMV) cell\u2010free DNA (cfDNA) fragments in women pregnant with a fetus with symptomatic congenital CMV (cCMV).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The study comprised nine women whose fetuses were diagnosed with cCMV between June 2019 and July 2024 at 20\u2009+\u20094 to 34\u2009+\u20091\u2009weeks' gestation (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098) or neonatally (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091) after primary or non\u2010primary maternal infection. In eight women, cfDNA sequencing data from a single timepoint were analyzed, either retrospectively, on data generated from 11\u201313\u2009weeks' gestation (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095) or prospectively, on data generated from 20\u201326\u2009weeks' gestation (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20093), upon the diagnosis of cCMV. In one woman (Case\u20096), CMV\u2010cfDNA analysis was performed at four timepoints: at 12\u2009+\u20095\u2009weeks (routine non\u2010invasive prenatal testing); 23\u2009+\u20093\u2009weeks (cCMV diagnosis); and 30\u2009min and 12\u2009h after termination of pregnancy (TOP) at 23\u2009+\u20096\u2009weeks.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CMV\u2010cfDNA was detectable in all cases. Mostly low levels of CMV\u2010cfDNA were observed in samples obtained at 11\u201313\u2009weeks' gestation and consistently high levels of CMV\u2010cfDNA were present in samples obtained at cCMV diagnosis. In Case\u20096, the level of maternal CMV\u2010cfDNA decreased substantially in the samples tested after TOP, compared with samples obtained before TOP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Low levels of CMV\u2010cfDNA detected between 11 and 13\u2009weeks may be a biomarker for severe fetal cCMV. CMV\u2010cfDNA analysis in the first trimester could be of added value in CMV screening, particularly for non\u2010primary maternal infections that cannot be identified using other methods. However, as CMV\u2010cfDNA is detectable in many pregnant women in the first trimester, further studies are needed to determine the predictive value of CMV\u2010cfDNA as a biomarker for the development of severe fetal cCMV. High levels of CMV\u2010cfDNA at fetal cCMV diagnosis and low levels directly after TOP suggest that the level of CMV\u2010cfDNA in maternal plasma may not necessarily reflect an active maternal infection, but could indicate a placental infection. \u00a9 2025 The Author(s).\n                      <jats:italic>Ultrasound in Obstetrics &amp; Gynecology<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/uog.29199","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T17:39:05Z","timestamp":1741714745000},"page":"470-477","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Detection of human cytomegalovirus cell\u2010free\n                    <scp>DNA<\/scp>\n                    in pregnant women with symptomatically infected fetuses: proof\u2010of\u2010concept study"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2311-7725","authenticated-orcid":false,"given":"B. H. W.","family":"Faas","sequence":"first","affiliation":[{"name":"Department of Human Genetics Radboud university medical center Nijmegen  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Meuleman","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Radboud university medical center Nijmegen  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Astuti","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Radboud university medical center Nijmegen  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Reuss","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Radboud university medical center Nijmegen  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Stol","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Amalia Children's Hospital Radboud university medical center Nijmegen  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. A.","family":"Sistermans","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Amsterdam UMC location Vrije Universiteit Amsterdam  Amsterdam The Netherlands"},{"name":"Amsterdam Reproduction &amp; Development research institute, Amsterdam UMC  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Linthorst","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Amsterdam UMC location Vrije Universiteit Amsterdam  Amsterdam The Netherlands"},{"name":"Amsterdam Reproduction &amp; Development research institute, Amsterdam UMC  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"van Leeuwen","sequence":"additional","affiliation":[{"name":"Amsterdam Reproduction &amp; Development research institute, Amsterdam UMC  Amsterdam The Netherlands"},{"name":"Department of Obstetrics Amsterdam UMC location University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Rahamat\u2010Langendoen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Radboud university medical center Nijmegen  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. A.","family":"Wilmink","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Radboud university medical center Nijmegen  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2016.02.042"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)31868-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.23685"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.26039"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad230"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanepe.2024.100892"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22414-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-60655-6"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.6143"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2024.104983"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2019.10.005"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.05.038"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02894070"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(71)80147-6"},{"key":"e_1_2_7_16_1","first-page":"403","article-title":"Placental evidence of cytomegalovirus infection of the fetus and neonate","volume":"108","author":"Mostoufi\u2010zadeh M","year":"1984","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2003.09.012"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.9.7598-7602.1998"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10070383"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.26226"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.535"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1744"}],"container-title":["Ultrasound in Obstetrics &amp; Gynecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/uog.29199","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T17:44:20Z","timestamp":1751564660000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/uog.29199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,3]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/uog.29199"],"URL":"https:\/\/doi.org\/10.1002\/uog.29199","archive":["Portico"],"relation":{},"ISSN":["0960-7692","1469-0705"],"issn-type":[{"value":"0960-7692","type":"print"},{"value":"1469-0705","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,3]]},"assertion":[{"value":"2024-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}