{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T22:28:47Z","timestamp":1783031327386,"version":"3.54.6"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T00:00:00Z","timestamp":1742774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010809","name":"Jascha Fonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006197","name":"Fonden til L\u00e6gevidenskabens Fremme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006197","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008269","name":"L\u00e6ge Sofus Carl Emil Friis og Hustru Olga Doris Friis' Legat","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ultrasound in Obstet &amp; Gyne"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess the value of pregnancy\u2010associated plasma protein\u2010A (PAPP\u2010A) in screening for preterm pre\u2010eclampsia (PE) (delivery &lt;\u200937\u2009weeks' gestation) measured in maternal blood samples collected before 11\u2009weeks, and to compare the screening performance of PAPP\u2010A with that of placental growth factor (PlGF) from blood samples collected at 8\u201314\u2009weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study analyzed data from women who participated in the PRESIDE (Pre\u2010eclampsia Screening in Denmark) study, a prospective, non\u2010interventional multicenter study investigating the predictive performance of the Fetal Medicine Foundation first\u2010trimester screening algorithm for PE in a Danish population. As part of combined first\u2010trimester screening, a routine blood sample was collected at 8\u201314\u2009weeks' gestation and PAPP\u2010A was measured. Excess serum was stored at \u221280\u00b0C and analyzed for PlGF in batches after delivery. Most women in the PRESIDE study had an extra blood sample collected at the time of the first\u2010trimester scan at 11\u201314\u2009weeks, which was also analyzed for PlGF and PAPP\u2010A in batches after all the participants had delivered. Screening performance was assessed in terms of  the detection rate at a 10% screen\u2010positive rate (SPR) for a combination of PAPP\u2010A or PlGF with maternal factors alone and for a combination of each of these biomarkers with maternal factors, mean arterial pressure (MAP) and uterine artery pulsatility index (UtA\u2010PI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study population comprised 8386 women who had a routine combined first\u2010trimester aneuploidy screening blood sample collected at 8\u201314\u2009weeks' gestation. In pregnancies that developed preterm PE, the median PAPP\u2010A multiples of the median from routine blood samples were 0.78 (95%\u2009CI, 0.67\u20130.90) before 10\u2009weeks, 0.80 (95%\u2009CI, 0.58\u20131.10) at 10\u2009weeks and 0.64 (95%\u2009CI, 0.53\u20130.78) at 11\u201314\u2009weeks. In women with samples collected before 10\u2009weeks, there was no significant improvement in the detection rate of preterm PE when PAPP\u2010A or PlGF was combined with maternal factors alone or when combined with maternal factors, MAP and UtA\u2010PI. In routine samples collected at or after 10\u2009weeks, PAPP\u2010A only increased the detection rate of preterm PE slightly. However, PlGF in samples collected at or after 10\u2009weeks increased the detection rate from 31.3% (95%\u2009CI, 16.1\u201350.0%) to 56.3% (95%\u2009CI, 37.7\u201373.6%) at a 10% SPR, i.e. an increase in the detection rate of 25.0% (95%\u2009CI, 4.3\u201344.4%), when combined with maternal factors alone. When PlGF collected from the PRESIDE sample at 11\u201314\u2009weeks was combined with maternal factors, MAP and UtA\u2010PI, there was an increase in the detection rate from 50.9% (95%\u2009CI, 37.1\u201364.6%) to 67.3% (95%\u2009CI, 53.3\u201379.3%), i.e. an increase of 16.4% (95%\u2009CI, 5.6\u201329.0%) at a 10% SPR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PAPP\u2010A has limited value in first\u2010trimester screening for PE, whereas PlGF adds significantly to the detection rate of preterm PE at 10\u201314\u2009weeks' gestation. \u00a9 2025 The Author(s). <jats:italic>Ultrasound in Obstetrics &amp; Gynecology<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/uog.29204","type":"journal-article","created":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T22:45:01Z","timestamp":1742942701000},"page":"567-574","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Screening for pre\u2010eclampsia using pregnancy\u2010associated plasma protein\u2010A or placental growth factor measurements in blood samples collected at 8\u201314 weeks' gestation"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9080-3389","authenticated-orcid":false,"given":"L.","family":"Rode","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, Rigshospitalet Copenhagen University Hospital  Copenhagen Denmark"},{"name":"Center for Fetal Medicine and Pregnancy, Department of Gynecology, Fertility, and Obstetrics, Rigshospitalet Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Wright","sequence":"additional","affiliation":[{"name":"Institute of Health Research University of Exeter  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Wright","sequence":"additional","affiliation":[{"name":"Institute of Health Research University of Exeter  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Overgaard","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Odense University Hospital  Odense Denmark"},{"name":"Department of Clinical Research University of Southern Denmark  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Sperling","sequence":"additional","affiliation":[{"name":"Fetal Medicine Unit, Department of Obstetrics and Gynecology Odense University Hospital  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Sandager","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Center for Fetal Medicine Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Aarhus Denmark"},{"name":"Center for Fetal Diagnostics Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"N\u00f8rgaard","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Copenhagen University Hospital North Zealand  Hiller\u00f8d Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. S.","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Fetal Medicine Unit, Department of Obstetrics and Gynecology Copenhagen University Hospital Hvidovre  Hvidovre Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Zingenberg","sequence":"additional","affiliation":[{"name":"Fetal Medicine Unit, Department of Obstetrics and Gynecology Copenhagen University Hospital Herlev and Gentofte  Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9353-8958","authenticated-orcid":false,"given":"I.","family":"Riishede","sequence":"additional","affiliation":[{"name":"Center for Fetal Medicine and Pregnancy, Department of Gynecology, Fertility, and Obstetrics, Rigshospitalet Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Tabor","sequence":"additional","affiliation":[{"name":"Center for Fetal Medicine and Pregnancy, Department of Gynecology, Fertility, and Obstetrics, Rigshospitalet Copenhagen University Hospital  Copenhagen Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. K.","family":"Ekelund","sequence":"additional","affiliation":[{"name":"Center for Fetal Medicine and Pregnancy, Department of Gynecology, Fertility, and Obstetrics, Rigshospitalet Copenhagen University Hospital  Copenhagen Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the PRESIDE Study Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.1860"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.6412"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.7755"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0705.2002.00808.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2057"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.12266"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.949"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1258\/096914103321824133"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000341264"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2015.02.018"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2019.11.1247"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2015.08.034"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.22071"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.01.020"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.15982"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.15750"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2010.02.010"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa031884"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwq373"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.27462"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.26183"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.12802"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/1460458218799505"},{"key":"e_1_2_7_25_1","unstructured":"The Fetal Medicine Foundation. Accessed October 27 2022.https:\/\/fetalmedicine.org\/research\/utpi"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.10803"},{"key":"e_1_2_7_27_1","volume-title":"A language and environment for statistical computing","author":"R Development Core Team","year":"2013"},{"key":"e_1_2_7_28_1","unstructured":"SchererR.PropCIs: Various Confidence Interval Methods for Proportions. Accessed March 5 2023.https:\/\/CRAN.R\u2010project.org\/package=PropCIs"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.26233"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.23669"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.10.035"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.11173"}],"container-title":["Ultrasound in Obstetrics &amp; Gynecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/uog.29204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T04:16:41Z","timestamp":1755749801000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/uog.29204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,24]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/uog.29204"],"URL":"https:\/\/doi.org\/10.1002\/uog.29204","archive":["Portico"],"relation":{},"ISSN":["0960-7692","1469-0705"],"issn-type":[{"value":"0960-7692","type":"print"},{"value":"1469-0705","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,24]]},"assertion":[{"value":"2024-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}